Targeted long-read sequencing reveals clonally expanded HBV-associated chromosomal translocations in patients with chronic hepatitis B - van Buuren, Nicholas; Ramirez, Ricardo; Soulette, Cameron; Suri, Vithika; Han, Dong; May, Lindsey; Turner, Scott; Parvangada, P.C.; Martin, Ross; Chan, Henry L.Y.; Marcellin, Patrick; Buti, Maria; et al. (JHEP reports, 2022)
Keywords: Chromosomal translocations; Chronic HBV; Clonal expansion; Gastroenterology and Hepatology; Integrated HBV DNA; Long-read sequencing; Targeted sequencing; N/A; DNA-Seq; WGS; cccDNA; Long-read sequencing; Integrated HBV DNA; PEG-IFNα; Clonal expansion; rcDNA; TERT; FFPE; TDF; gDNA; DR1; HCC; Chronic HBV; contig; dslDNA; CCS; pgRNA; RNA-Seq; RIN; Chromosomal translocations; targeted Iso-Seq; NHEJ; Targeted sequencing; CHB; circular consensus sequence; formalin-fixed paraffin-embedded; direct repeat 1; hepatocellular carcinoma; chronic hepatitis B; RNA sequencing; genomic DNA; nucleos(t)ide analogue; pegylated interferon α; RNA integrity number; tenofovir disoproxil fumarate; pre-genomic RNA; whole genome sequencing; non-homologous end-joining; targeted long-read RNA-sequencing; contiguous sequence; telomerase reverse transcriptase; relaxed circular DNA; covalently closed circular DNA; DNA sequencing; double-stranded linear DNA; contig, contiguous sequence; N/A, nucleos(t)ide analogue; gDNA, genomic DNA; DR1, direct repeat 1; PEG-IFNα, pegylated interferon α; CHB, chronic hepatitis B; pgRNA, pre-genomic RNA; RIN, RNA integrity number; DNA-Seq, DNA sequencing; FFPE, formalin-fixed paraffin-embedded; NHEJ, non-homologous end-joining; TERT, telomerase reverse transcriptase; targeted Iso-Seq, targeted long-read RNA-sequencing; RNA-Seq, RNA sequencing; WGS, whole genome sequencing; dslDNA, double-stranded linear DNA; CCS, circular consensus sequence; TDF, tenofovir disoproxil fumarate; HCC, hepatocellular carcinoma; cccDNA, covalently closed circular DNA; rcDNA, relaxed circular DNA