A Secondary Structure in the 5' Untranslated Region of adhE mRNA Required for RNase G-Dependent Regulation

Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively. Previous research indicates that the 5' untranslated region (5'-UTR) of adhE mRNA gives RNase G-dependency to lacZ mRNA when tagged...

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Published inBioscience, biotechnology, and biochemistry Vol. 77; no. 12; pp. 2473 - 2479
Main Authors ITO, Kazutaka, HAMASAKI, Kohshin, KAYAMORI, Aya, NGUYEN, Phuong Anh Thi, AMAGAI, Kaoru, WACHI, Masaaki
Format Journal Article
LanguageEnglish
Published England Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013
Oxford University Press
Subjects
5' Untranslated Regions - genetics
5'-UTR
adhE
Alcohol Dehydrogenase - genetics
Aldehyde Oxidoreductases - genetics
Base Sequence
Binding Sites
Endoribonucleases - metabolism
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Gene Expression Regulation, Bacterial
Lac Operon - genetics
mRNA stability
Nucleic Acid Conformation
Protein Binding
RNase G
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Abstract Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively. Previous research indicates that the 5' untranslated region (5'-UTR) of adhE mRNA gives RNase G-dependency to lacZ mRNA when tagged at the 5'-end, but it has not been elucidated yet how RNase G recognizes adhE mRNA. Primer extension analysis revealed that RNase G cleaved a phosphodiester bond between −19A and −18C in the 5'-UTR (the A of the start codon was defined as +1). Site-directed mutagenesis indicated that RNase G did not recognize the nucleotides at −19 and −18. Random deletion analysis indicated that the sequence from −145 to −125 was required for RNase G-dependent degradation. Secondary structure prediction and further site-directed deletion suggested that the stem-loop structure, with a bubble in the stem, is required for RNaseG-dependent degradation of adhE mRNA.
AbstractList Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively. Previous research indicates that the 5' untranslated region (5'-UTR) of adhE mRNA gives RNase G-dependency to lacZ mRNA when tagged at the 5'-end, but it has not been elucidated yet how RNase G recognizes adhE mRNA. Primer extension analysis revealed that RNase G cleaved a phosphodiester bond between -19A and -18C in the 5'-UTR (the A of the start codon was defined as +1). Site-directed mutagenesis indicated that RNase G did not recognize the nucleotides at -19 and -18. Random deletion analysis indicated that the sequence from -145 to -125 was required for RNase G-dependent degradation. Secondary structure prediction and further site-directed deletion suggested that the stem-loop structure, with a bubble in the stem, is required for RNaseG-dependent degradation of adhE mRNA.
Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively. Previous research indicates that the 5' untranslated region (5'-UTR) of adhE mRNA gives RNase G-dependency to lacZ mRNA when tagged at the 5'-end, but it has not been elucidated yet how RNase G recognizes adhE mRNA. Primer extension analysis revealed that RNase G cleaved a phosphodiester bond between −19A and −18C in the 5'-UTR (the A of the start codon was defined as +1). Site-directed mutagenesis indicated that RNase G did not recognize the nucleotides at −19 and −18. Random deletion analysis indicated that the sequence from −145 to −125 was required for RNase G-dependent degradation. Secondary structure prediction and further site-directed deletion suggested that the stem-loop structure, with a bubble in the stem, is required for RNaseG-dependent degradation of adhE mRNA.
Author WACHI, Masaaki
KAYAMORI, Aya
AMAGAI, Kaoru
HAMASAKI, Kohshin
ITO, Kazutaka
NGUYEN, Phuong Anh Thi
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Snippet Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively....
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SubjectTerms 5' Untranslated Regions - genetics
5'-UTR
adhE
Alcohol Dehydrogenase - genetics
Aldehyde Oxidoreductases - genetics
Base Sequence
Binding Sites
Endoribonucleases - metabolism
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Gene Expression Regulation, Bacterial
Lac Operon - genetics
mRNA stability
Nucleic Acid Conformation
Protein Binding
RNase G
Title A Secondary Structure in the 5' Untranslated Region of adhE mRNA Required for RNase G-Dependent Regulation
URI https://www.tandfonline.com/doi/abs/10.1271/bbb.130618
https://www.ncbi.nlm.nih.gov/pubmed/24317071
https://www.proquest.com/docview/1478089088/abstract/
https://search.proquest.com/docview/1647010089
Volume 77
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