Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis

• Published in: FEBS open bio Vol. 6; no. 2; pp. 135 - 146
• Main Authors: Vishwakarma, Abhaypratap, Dalal, Ahan, Tetali, Sarada Devi, Kirti, Pulugurtha Bharadwaja, Padmasree, Kollipara
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.02.2016; John Wiley and Sons Inc; Wiley
• Subjects: Abiotic stress; Alternative oxidase; alternative oxidase 1a; Amino acids; Antioxidants; Apoptosis; Cyanides; Cytochrome; E coli; Enzymes; Genetic engineering; Homeostasis; NAD; NADH; Organisms; Oxidative stress; Reactive oxygen species; redox homeostasis; Respiration; Saccharomyces cerevisiae; Scholarships & fellowships; Yeast; United States > US; redox homeostasis; respiration; alternative oxidase 1a; Saccharomyces cerevisiae; oxidative stress; reactive oxygen species
• ISSN: 2211-5463; 2211-5463
• DOI: 10.1002/2211-5463.12028

This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)‐sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD+/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.