Double-negative feedback loop between long non-coding RNA TUG1 and miR-145 promotes epithelial to mesenchymal transition and radioresistance in human bladder cancer cells

• Published in: FEBS letters Vol. 589; no. 20; pp. 3175 - 3181
• Main Authors: Tan, Jiemei, Qiu, Kaifeng, Li, Mingyi, Liang, Ying
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 07.10.2015
• Subjects: 3' Untranslated Regions - genetics; Aged; Aged, 80 and over; Base Sequence; Bladder cancer; Blotting, Western; Cell Line; Cell Line, Tumor; Epithelial-Mesenchymal Transition - genetics; Epithelial-to-mesenchymal transition; epithelium; Feedback, Physiological; Female; Gene Expression Regulation, Neoplastic; HEK293 Cells; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Humans; Male; metastasis; MicroRNAs - genetics; Middle Aged; miR-145; neoplasm cells; non-coding RNA; radiation resistance; Radiation Tolerance - genetics; Radioresistance; Repressor Proteins - genetics; Repressor Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Sequence Homology, Nucleic Acid; therapeutics; Transplantation, Heterologous; TUG1; urinary bladder neoplasms; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology; ZEB2; Zinc Finger E-box Binding Homeobox 2; Radioresistance; ZEB2; TUG1; Bladder cancer; Epithelial-to-mesenchymal transition; miR-145

•TUG1 promoted bladder cancer cell invasion and radioresistance through inducing epithelial-to-mesenchymal transition (EMT).•TUG1 decreased the expression of miR-145 and there was a reciprocal repression between TUG1 and miR-145 in an Argonaute2-dependent manner.•TUG1 exerted its function through th...