P204/正辛醇反胶束萃取L-辛弗林的研究

目的建立一种反胶束萃取L-辛弗林的方法,并验证利用P204/正辛醇萃取L-辛弗林的有效性。方法制备P204/正辛醇反胶束体系萃取枳实L-辛弗林,反萃取液经浓缩干燥后,获得纯化L-辛弗林。利用基于单因素实验进行的正交实验研究最佳分离工艺,通过紫外分析、原子力显微镜表征证实了P204/正辛醇反胶束体系萃取分离L-辛弗林的有效性。结果萃取最佳工艺条件为：水相pH值6.5、萃取时间15 min、P204浓度0.10mol/L、W_0=5,此条件下L-辛弗林的单次萃取率为68.02%,各影响因素影响顺序为：水相pH〉P204浓度〉W_0〉萃取时间。纯化后的L-辛弗林纯度达到87.8%,比原料纯度提升了2...

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Published in食品安全质量检测学报 Vol. 7; no. 4; pp. 1426 - 1431
Main Author 桑鲁燕 陶仁友 周小华 王亚文
Format Journal Article
LanguageChinese
Published 菏泽出入境检验检疫局,菏泽,274000%重庆大学化学化工学院,重庆,400044 2016
Subjects
L-辛弗林
反胶束
萃取
L-辛弗林
萃取
反胶束
reverse micellar
extraction
L-synephrine
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ISSN2095-0381

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Summary:目的建立一种反胶束萃取L-辛弗林的方法,并验证利用P204/正辛醇萃取L-辛弗林的有效性。方法制备P204/正辛醇反胶束体系萃取枳实L-辛弗林,反萃取液经浓缩干燥后,获得纯化L-辛弗林。利用基于单因素实验进行的正交实验研究最佳分离工艺,通过紫外分析、原子力显微镜表征证实了P204/正辛醇反胶束体系萃取分离L-辛弗林的有效性。结果萃取最佳工艺条件为：水相pH值6.5、萃取时间15 min、P204浓度0.10mol/L、W_0=5,此条件下L-辛弗林的单次萃取率为68.02%,各影响因素影响顺序为：水相pH〉P204浓度〉W_0〉萃取时间。纯化后的L-辛弗林纯度达到87.8%,比原料纯度提升了2.9倍。结论该方法为L-辛弗林的分离提取提供一种快速低成本方法,并对其萃取机制及主要变化规律方面提供了基础数据。
Bibliography:L-synephrine; reverse micellar; extraction
Objective To establish a method of reverse micelle extraction for L-synephrine, and prove the efficiency of extraction by P204/octanol reverse micelle. Methods P204/octanol was chosen and prepared for extracting L-synephrine, which was extracted from fructus. Purified L-synephrine was prepared after reverse extraction, concentration and drying. The optimal conditions of extraction were studied by orthogonal experiment based on single factor experiment. The efficiency of extraction by P204/octanol reverse micelle was proved by UV and AFM spectrum. Results The optimum conditions of extraction were as follows： p H of 6.5, extraction time of 15 min, P204 concentration of 0.10 mol/L, and W_0 of 5. The extraction rate was 68.02% at the optimum conditions for one time, and the effecting order was pHP〉204 concentration〉W0〉extraction time. The purified L-synephrine in reverse extraction was 87.8% after concentration and drying, which was 2.9 times as the raw material. Conclusi
ISSN:2095-0381