Direct detection of Corynebacterium striatum, Corynebacterium propinquum, and Corynebacterium simulans in sputum samples by high-resolution melt curve analysis

• Published in: BMC infectious diseases Vol. 21; no. 1; p. 21
• Main Authors: Xu, Shuai, Qiu, Xiaotong, Hou, Xuexin, Zhou, Haijian, Chen, Dongke, Wang, Xuebing, Han, Lichao, Li, Dan, Sun, Lina, Ji, Xingzhao, Li, Minghui, Zhang, Jingshan, Li, Mengtong, Li, Zhenjun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.01.2021; BioMed Central; BMC
• Subjects: Assaying; Bacterial identification; Bacterial Proteins - genetics; Clinical isolates; Confidence intervals; Corynebacteria; Corynebacterium; Corynebacterium - genetics; Corynebacterium - isolation & purification; Corynebacterium Infections - diagnosis; Corynebacterium Infections - microbiology; Corynebacterium species; Deoxyribonucleic acid; Diagnostic software; Diagnostic systems; Diagnostics; DNA; DNA Primers - genetics; Epidemiology; Genetic diversity; Genotyping Techniques - methods; High resolution; High-resolution melt curve analysis; Humans; Identification and classification; Laboratories; Limit of Detection; Mass spectrometry; Melting; Melting curve; Methods; Microbiological assay; Microorganisms; Nosocomial infections; Pathogens; Polymerase chain reaction; Real-Time Polymerase Chain Reaction - methods; Scientific imaging; Software; Species; Sputum; Sputum - microbiology; Testing; Varieties; China; France; United States > US; Germany; Diagnostics; High-resolution melt curve analysis; Bacterial identification; Corynebacterium species

Pulmonary infections caused by non-diphtheriae corynebacteria are increasing. However, rapid identification of Corynebacterium species poses a challenge due to the low genetic variation within the genus. Three reference strains and 99 clinical isolates were used in this study. A qPCR followed by hig...