A molecularly engineered split reporter for imaging protein-protein interactions with positron emission tomography

• Published in: Nature medicine Vol. 16; no. 8; pp. 921 - 926
• Main Authors: Massoud, Tarik F, Paulmurugan, Ramasamy, Gambhir, Sanjiv S
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.08.2010; Nature Publishing Group
• Subjects: 631/1647/245/2092; 631/1647/338; 631/1647/767/1424; 631/45/475/2290; Animal diseases; Animal models; Animals; Biomedical and Life Sciences; Biomedicine; Cancer Research; Cells, Cultured; Cloning, Molecular - methods; Emissions; Estrogens; Genes, Reporter - physiology; Genetic engineering; Herpes simplex virus 1; Humans; Hypoxia; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Infectious Diseases; Mammals; Medical imaging; Metabolic Diseases; Mice; Models, Biological; Molecular biology; Molecular Medicine; Mutation; Neurosciences; Observations; Peptide Fragments - genetics; Peptide Fragments - metabolism; PET imaging; Point Mutation - physiology; Positron-Emission Tomography - methods; Protein Binding; Protein Engineering - methods; Protein Interaction Mapping - methods; Protein-protein interactions; Proteins; Rodents; Stem cells; Tacrolimus Binding Protein 1A - metabolism; Tacrolimus Binding Proteins - metabolism; technical-report; Thymidine Kinase - chemistry; Thymidine Kinase - genetics; Thymidine Kinase - metabolism; Tomography; Transplantation, Heterologous; Von Hippel-Lindau Tumor Suppressor Protein - metabolism; United States

Tarik Massoud and colleagues offer a new, noninvasive molecular imaging technique based on split reporter complementation for quantifying and imaging protein-protein interactions—cytoplasmic and nuclear— in vivo using positron emission tomography. They use a split reporter system based on the enzyme...