Detection of Nipah virus in Pteropus medius in 2019 outbreak from Ernakulam district, Kerala, India
In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month. Specialized techniques w...
Saved in:
| Published in | BMC infectious diseases Vol. 21; no. 1; pp. 162 - 7 |
|---|---|
| Main Authors | , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
BioMed Central Ltd
09.02.2021
BioMed Central BMC |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month.
Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats.
One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats.
A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection. |
|---|---|
| AbstractList | In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month.BACKGROUNDIn June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month.Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats.METHODSSpecialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats.One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats.RESULTSOne rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats.A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection.CONCLUSIONA distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection. Background In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month. Methods Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats. Results One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats. Conclusion A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection. In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month. Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats. One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats. A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection. Background In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month. Methods Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats. Results One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats. Conclusion A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection. Keywords: Ernakulum, Nipah virus, NGS, IgG ELISA, Pteropus spp In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month. Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats. One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats. A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection. Abstract Background In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine if NiV was in circulation in Pteropus species (spp) in those areas where the index case had visit history in 1 month. Methods Specialized techniques were used to trap the Pteropus medius bats (random sampling) in the vicinity of the index case area. Throat and rectal swabs samples of 141 bats along with visceral organs of 92 bats were collected to detect the presence of NiV by real-time reverse transcriptase-polymerase chain reaction (qRTPCR). Serum samples of 52 bats were tested for anti-NiV Immunoglobulin (Ig) G antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). The complete genome of NiV was sequenced by next-generation sequencing (NGS) from the tissues and swab samples of bats. Results One rectal swab sample and three bats visceral organs were found positive for the NiV. Interestingly, 20.68% (12/58) of Pteropus were positive for anti-NiV IgG antibodies. NiV sequences of 18,172; 17,200 and 15,100 nucleotide bps could be retrieved from three Pteropus bats. Conclusion A distinct cluster of NiV sequences, with significant net-evolutionary nucleotide divergence, was obtained, suggesting the circulation of new genotype (I-India) in South India. NiV Positivity in Pteropus spp. of bats revealed that NiV is circulating in many districts of Kerala state, and active surveillance of NiV should be immediately set up to know the hotspot area for NiV infection. |
| ArticleNumber | 162 |
| Audience | Academic |
| Author | Balasubramanian, R. Patil, Savita Nyayanit, Dimpal A. Sudeep, A. B. Gokhale, Mangesh D. Majumdar, Triparna D. Yadav, Pragya D. Patil, Dilip R. Sugunan, A. P. Sahay, Rima R. Pardeshi, Prachi G. Mourya, Devendra T. Jain, Rajlaxmi Shete, Anita Gupta, Nivedita Gopale, Sanjay |
| Author_xml | – sequence: 1 givenname: A. B. surname: Sudeep fullname: Sudeep, A. B. – sequence: 2 givenname: Pragya D. surname: Yadav fullname: Yadav, Pragya D. – sequence: 3 givenname: Mangesh D. surname: Gokhale fullname: Gokhale, Mangesh D. – sequence: 4 givenname: R. surname: Balasubramanian fullname: Balasubramanian, R. – sequence: 5 givenname: Nivedita surname: Gupta fullname: Gupta, Nivedita – sequence: 6 givenname: Anita surname: Shete fullname: Shete, Anita – sequence: 7 givenname: Rajlaxmi surname: Jain fullname: Jain, Rajlaxmi – sequence: 8 givenname: Savita surname: Patil fullname: Patil, Savita – sequence: 9 givenname: Rima R. surname: Sahay fullname: Sahay, Rima R. – sequence: 10 givenname: Dimpal A. surname: Nyayanit fullname: Nyayanit, Dimpal A. – sequence: 11 givenname: Sanjay surname: Gopale fullname: Gopale, Sanjay – sequence: 12 givenname: Prachi G. surname: Pardeshi fullname: Pardeshi, Prachi G. – sequence: 13 givenname: Triparna D. surname: Majumdar fullname: Majumdar, Triparna D. – sequence: 14 givenname: Dilip R. surname: Patil fullname: Patil, Dilip R. – sequence: 15 givenname: A. P. surname: Sugunan fullname: Sugunan, A. P. – sequence: 16 givenname: Devendra T. surname: Mourya fullname: Mourya, Devendra T. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33563231$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNkktv1DAUhSNURB_wB1ggS2xAaoofcWxvkKpSYERFEa-t5djO1NMkHmyngn-PZzJFTYUQ8sLW9XeOfa_OYbE3-MEWxVMETxDi9auIMGeihBiVkPKaluxBcYAqhkpMSLV357xfHMa4ghAxjsWjYp8QWhNM0EGh39hkdXJ-AL4FH91aXYEbF8YI3AA-JRv8Op97a9xUwhAJ4MfUBKuuQRt8D87DoK7HTvXAuJiC0-kYfLBBdeoYLAbj1OPiYau6aJ_s9qPi29vzr2fvy4vLd4uz04tS1wSlkhgksOLWktpyZihFBhtaN5pY0kJoaFtTajRtFBGCcKZJQzTjxFKEG6EVOSoWk6_xaiXXwfUq_JJeObkt-LCUKiSnOysrAbESUFdI6Io3iHOOIRa4gbrJD1TZ6_XktR6b3L22Q8odzUznN4O7kkt_IxlniDKSDV7sDIL_MdqYZO-itl2nBuvHKHHFORKQC5jR5_fQlR_zULsNJaCoBdsa7qilyg24ofX5Xb0xlac1JZTVFUWZOvkLlZexvdM5P63L9Zng5UyQmWR_pqUaY5SLL5__n738Pmef3R3gn8ndRi8DfAJ08DEG20rtktokMf_YdRJBuUm5nFIuc8rlNuWSZSm-J711_4foN2No-iA |
| CitedBy_id | crossref_primary_10_3390_v17010081 crossref_primary_10_1016_j_jiph_2021_05_001 crossref_primary_10_1371_journal_pgph_0003926 crossref_primary_10_3390_v15122407 crossref_primary_10_3389_fpubh_2022_818545 crossref_primary_10_48022_mbl_2404_04012 crossref_primary_10_1002_jmv_29559 crossref_primary_10_4103_ijmr_IJMR_4388_20 crossref_primary_10_1016_j_cimid_2024_102183 crossref_primary_10_3389_fmicb_2024_1493428 crossref_primary_10_1097_IM9_0000000000000165 crossref_primary_10_3389_fmicb_2021_811157 crossref_primary_10_1007_s11684_024_1078_2 crossref_primary_10_1097_MS9_0000000000001057 crossref_primary_10_3390_immuno3020011 crossref_primary_10_1016_j_ebiom_2022_104405 crossref_primary_10_1016_j_cimid_2022_101800 crossref_primary_10_1002_hsr2_70195 crossref_primary_10_1016_j_bbi_2021_09_015 crossref_primary_10_3389_fpubh_2024_1356515 crossref_primary_10_22207_JPAM_16_1_72 crossref_primary_10_1016_j_clinpr_2024_100390 crossref_primary_10_33590_emj_ILFC3139 crossref_primary_10_3389_fmicb_2024_1342170 crossref_primary_10_1016_j_coviro_2021_04_013 crossref_primary_10_1080_21505594_2023_2224642 crossref_primary_10_5334_aogh_3431 |
| Cites_doi | 10.1016/S1386-6532(02)00268-8 10.1038/s41598-019-40476-y 10.3201/eid1612.091790 10.1016/j.jviromet.2004.05.018 10.4103/ijmr.IJMR_1488_16 10.1093/molbev/msw054 10.3201/eid1705.100968 10.1126/science.288.5470.1432 10.3201/eid1202.051247 10.1136/bmjgh-2018-001118 10.3201/eid2405.171844 10.3201/eid2506.181620 10.1093/cid/ciy1093 10.3201/eid2505.181076 10.1007/s12250-018-0050-1 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2021 BioMed Central Ltd. 2021. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. The Author(s) 2021 |
| Copyright_xml | – notice: COPYRIGHT 2021 BioMed Central Ltd. – notice: 2021. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: The Author(s) 2021 |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM IOV ISR 3V. 7QL 7T2 7U9 7X7 7XB 88E 8C1 8FI 8FJ 8FK ABUWG AEUYN AFKRA AZQEC BENPR C1K CCPQU DWQXO FYUFA GHDGH H94 K9. M0S M1P M7N PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQQKQ PQUKI 7X8 5PM DOA |
| DOI | 10.1186/s12879-021-05865-7 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Bacteriology Abstracts (Microbiology B) Health and Safety Science Abstracts (Full archive) Virology and AIDS Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Public Health Database Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest Central Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Korea Health Research Premium Collection Health Research Premium Collection (Alumni) AIDS and Cancer Research Abstracts ProQuest Health & Medical Complete (Alumni) ProQuest Health & Medical Collection Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing Environmental Sciences and Pollution Management ProQuest Central ProQuest One Sustainability Health Research Premium Collection Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Health & Medical Research Collection AIDS and Cancer Research Abstracts Health & Safety Science Abstracts ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Public Health Virology and AIDS Abstracts ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic Publicly Available Content Database MEDLINE |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ (Directory of Open Access Journals) url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1471-2334 |
| EndPage | 7 |
| ExternalDocumentID | oai_doaj_org_article_4902a90c419c48b188820292b0cb5ba4 PMC7871573 A653576451 33563231 10_1186_s12879_021_05865_7 |
| Genre | Journal Article |
| GeographicLocations | India Bangladesh Kerala India Malaysia Singapore United States--US |
| GeographicLocations_xml | – name: India – name: Singapore – name: Kerala India – name: Bangladesh – name: Malaysia – name: United States--US |
| GrantInformation_xml | – fundername: Indian Council of Medical Research grantid: - – fundername: ; grantid: - |
| GroupedDBID | --- 0R~ 23N 2WC 53G 5VS 6J9 6PF 7X7 88E 8C1 8FI 8FJ AAFWJ AAJSJ AASML AAWTL AAYXX ABDBF ABUWG ACGFO ACGFS ACIHN ACPRK ACUHS ADBBV ADRAZ ADUKV AEAQA AENEX AEUYN AFKRA AFPKN AFRAH AHBYD AHMBA AHYZX ALIPV ALMA_UNASSIGNED_HOLDINGS AMKLP AMTXH AOIJS BAPOH BAWUL BCNDV BENPR BFQNJ BMC BPHCQ BVXVI C6C CCPQU CITATION CS3 DIK DU5 E3Z EAD EAP EAS EBD EBLON EBS EMB EMK EMOBN ESX F5P FYUFA GROUPED_DOAJ GX1 HMCUK HYE IAO IHR INH INR IOV ISR ITC KQ8 M1P M48 M~E O5R O5S OK1 OVT P2P PGMZT PHGZM PHGZT PIMPY PQQKQ PROAC PSQYO RBZ RNS ROL RPM RSV SMD SOJ SV3 TR2 TUS UKHRP W2D WOQ WOW XSB -A0 3V. ACRMQ ADINQ C24 CGR CUY CVF ECM EIF NPM PMFND 7QL 7T2 7U9 7XB 8FK AZQEC C1K DWQXO H94 K9. M7N PJZUB PKEHL PPXIY PQEST PQUKI 7X8 5PM PUEGO |
| ID | FETCH-LOGICAL-c631t-3d192a8ee36e87d551d2d56bc3e3f00d5f655dc5ba399387c3b3c783e512b9ca3 |
| IEDL.DBID | M48 |
| ISSN | 1471-2334 |
| IngestDate | Wed Aug 27 01:30:20 EDT 2025 Thu Aug 21 14:02:33 EDT 2025 Thu Jul 10 17:16:31 EDT 2025 Sat Jul 26 00:18:34 EDT 2025 Tue Jun 17 21:29:05 EDT 2025 Tue Jun 10 20:40:32 EDT 2025 Fri Jun 27 04:19:22 EDT 2025 Fri Jun 27 03:42:00 EDT 2025 Thu Jan 02 22:57:21 EST 2025 Thu Apr 24 22:54:07 EDT 2025 Tue Jul 01 01:57:47 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | Nipah virus IgG ELISA NGS Ernakulum Pteropus spp |
| Language | English |
| License | Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c631t-3d192a8ee36e87d551d2d56bc3e3f00d5f655dc5ba399387c3b3c783e512b9ca3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.1186/s12879-021-05865-7 |
| PMID | 33563231 |
| PQID | 2490969773 |
| PQPubID | 42582 |
| PageCount | 7 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_4902a90c419c48b188820292b0cb5ba4 pubmedcentral_primary_oai_pubmedcentral_nih_gov_7871573 proquest_miscellaneous_2488190890 proquest_journals_2490969773 gale_infotracmisc_A653576451 gale_infotracacademiconefile_A653576451 gale_incontextgauss_ISR_A653576451 gale_incontextgauss_IOV_A653576451 pubmed_primary_33563231 crossref_citationtrail_10_1186_s12879_021_05865_7 crossref_primary_10_1186_s12879_021_05865_7 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2021-02-09 |
| PublicationDateYYYYMMDD | 2021-02-09 |
| PublicationDate_xml | – month: 02 year: 2021 text: 2021-02-09 day: 09 |
| PublicationDecade | 2020 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England – name: London |
| PublicationTitle | BMC infectious diseases |
| PublicationTitleAlternate | BMC Infect Dis |
| PublicationYear | 2021 |
| Publisher | BioMed Central Ltd BioMed Central BMC |
| Publisher_xml | – name: BioMed Central Ltd – name: BioMed Central – name: BMC |
| References | KB Chua (5865_CR1) 2003; 26 KB Chua (5865_CR2) 2000; 288 SB Kasloff (5865_CR16) 2019; 9 MS Chadha (5865_CR6) 2006; 12 PD Yadav (5865_CR12) 2018; 24 P Yadav (5865_CR10) 2018; 147 DT Mourya (5865_CR8) 2019; 69 PD Yadav (5865_CR11) 2019; 25 CE Mire (5865_CR14) 2019; 25 B Sun (5865_CR4) 2018; 33 S Kumar (5865_CR13) 2016; 33 SA Rahman (5865_CR15) 2010; 16 V Guillaume (5865_CR9) 2004; 120 LT Mazzola (5865_CR3) 2019; 4 P Yadav (5865_CR7) 2018; 147 VA Arankalle (5865_CR5) 2011; 17 |
| References_xml | – volume: 26 start-page: 265 year: 2003 ident: 5865_CR1 publication-title: J Clin Virol doi: 10.1016/S1386-6532(02)00268-8 – volume: 9 start-page: 5230 year: 2019 ident: 5865_CR16 publication-title: Sci Rep doi: 10.1038/s41598-019-40476-y – volume: 16 start-page: 1990 year: 2010 ident: 5865_CR15 publication-title: Emerg Infect Dis doi: 10.3201/eid1612.091790 – volume: 120 start-page: 229 year: 2004 ident: 5865_CR9 publication-title: J Virol Methods doi: 10.1016/j.jviromet.2004.05.018 – volume: 147 start-page: 318 year: 2018 ident: 5865_CR7 publication-title: Indian J Med Res doi: 10.4103/ijmr.IJMR_1488_16 – volume: 33 start-page: 1870 year: 2016 ident: 5865_CR13 publication-title: Mol Biol Evol doi: 10.1093/molbev/msw054 – volume: 17 start-page: 907 year: 2011 ident: 5865_CR5 publication-title: Emerg Infect Dis doi: 10.3201/eid1705.100968 – volume: 288 start-page: 1432 year: 2000 ident: 5865_CR2 publication-title: Science doi: 10.1126/science.288.5470.1432 – volume: 12 start-page: 235 year: 2006 ident: 5865_CR6 publication-title: Emerg Infect Dis doi: 10.3201/eid1202.051247 – volume: 4 year: 2019 ident: 5865_CR3 publication-title: BMJ Glob Health doi: 10.1136/bmjgh-2018-001118 – volume: 147 start-page: 318 year: 2018 ident: 5865_CR10 publication-title: Indian J Med Res doi: 10.4103/ijmr.IJMR_1488_16 – volume: 24 start-page: 898 year: 2018 ident: 5865_CR12 publication-title: Emerg Infect Dis doi: 10.3201/eid2405.171844 – volume: 25 start-page: 1144 year: 2019 ident: 5865_CR14 publication-title: Emerg Infect Dis doi: 10.3201/eid2506.181620 – volume: 69 start-page: 378 year: 2019 ident: 5865_CR8 publication-title: Clin Infect Dis doi: 10.1093/cid/ciy1093 – volume: 25 start-page: 1003 year: 2019 ident: 5865_CR11 publication-title: Emerg Infect Dis doi: 10.3201/eid2505.181076 – volume: 33 start-page: 385 year: 2018 ident: 5865_CR4 publication-title: Virol Sin doi: 10.1007/s12250-018-0050-1 |
| SSID | ssj0017829 |
| Score | 2.429219 |
| Snippet | In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to determine... Background In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was undertaken to... Abstract Background In June 2019, Nipah virus (NiV) infection was detected in a 21-year-old male (index case) of Ernakulum, Kerala, India. This study was... |
| SourceID | doaj pubmedcentral proquest gale pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 162 |
| SubjectTerms | Animals Antibodies Antibodies, Viral - blood Antigens Chiroptera Chiroptera - virology Disease hot spots Disease Outbreaks Distribution Divergence Enzyme-linked immunosorbent assay Ernakulum Genomes Genotypes Health aspects Henipavirus Infections - diagnosis Henipavirus Infections - epidemiology Henipavirus Infections - veterinary Henipavirus Infections - virology High-Throughput Nucleotide Sequencing Host-virus relationships IgG ELISA Immunoglobulin G Immunoglobulin G - blood India - epidemiology Infectious diseases Liver Methods Next-generation sequencing NGS Nipah virus Nipah Virus - classification Nipah Virus - genetics Nipah Virus - immunology Nucleotides Phylogenetics Phylogeny Polymerase chain reaction Pteropus Pteropus spp Random sampling Real-Time Polymerase Chain Reaction Rectum Rectum - virology RNA, Viral - chemistry RNA, Viral - metabolism RNA-directed DNA polymerase Sentinel health events Spleen Statistical sampling |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELZQD4gL4k2gRQYhcaBRk_h9LNCqBbUgoKg3y3acdlWUrJqkv5-ZJLvaCAEXblE8VpKZseebePyZkNdalgXi1lRH8GCehzx1XPEUoqGLKiihDG5OPjmVR2f847k43zjqC2vCRnrgUXF73GSFM1nguQlc-xwyNsjXTeGz4IV3AxMoxLxVMjWtH0DcM6stMlrutTALK5NiOUImtBSpmoWhga3_9zl5IyjNCyY3ItDhPXJ3go50f3zl--RWrB-Q2yfT4vhDEj7EbiisqmlT0dPF0l3Sm8V139JFTb-A_polXONi-ngLgrKhTd9BUuyuKO4zoQfwhKsenISWSKi7CN0u_YR_rdwuPa7BlR6Rs8OD7--P0ukMhTRIlncpKwHCOR0jk2CMEvBRWZRC-sAiq7KsFJUUogygSUQqWgXmWVCaRQAC3gTHHpOtuqnjU0JzHwJ3FQBcX3LIQ7SXUhvorLzW3JmE5CuV2jARjOM5Fz_tkGhoaUczWDCDHcxgVULervssR3qNv0q_Q0utJZEae7gBDmMnh7H_cpiEvEI7WyS_qLG65sL1bWuPP_-w-1IwyL-4yP8k9O3rTOjNJFQ18KHBTTsaQF1IqjWT3J5JwhAO8-aVz9lpCmkt5MWQXgI8Zwl5uW7GnlgWV8emRxmNiE6bLCFPRhddK4cxIRmg94SomfPOtDdvqReXA8E4TOK5UOzZ_1D3c3KnGMZdkWZmm2x1133cARzX-RfDkP0F4Dk_lA priority: 102 providerName: Directory of Open Access Journals – databaseName: Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3db9QwDI9gSIgXxDeFgQJC4oFVa5vvJzRg0wbaQMDQvUVJ2m6nofa4tvz92L3csQqxt6px1MZ27J8TxyHklZZlgbg11RVoMM9DnjqueAre0FUqKKEMHk4-PpGHp_zjTMziglsX0yrXNnE01GUbcI18F8IEQNuAVtjbxa8Ub43C3dV4hcZ1cgNLl6FWq9km4MrB-5n1QRktdzuwxcqkmJSQCS1FqibOaKzZ_69lvuSapmmTl_zQwR1yOwJIureS-F1yrWrukZvHcYv8Pgkfqn5Mr2poW9OT-cKd09_z5dDReUO_ABfbBTzjlvrqFbhmQ9uhh9DYXVA8bUL34QsXA6gKLbGs7jz0O_QTrl25HXrUgEI9IKcH-9_fH6bxJoU0SJb3KSsByDldVUyCSEpASWVRCukDq1idZaWopRBlEN4hXtEqMM-C0qwCOOBNcOwh2WrapnpMaO5D4K4GmOtLDtGI9lJqA52V15o7k5B8zVIbYplxvO3ipx3DDS3tSgwWxGBHMViVkDebPotVkY0rqd-hpDaUWCB7fNEuz2ycbxa0pXAmCzw3gWufQ6BfZIUpfBY8DJMn5CXK2WIJjAZzbM7c0HX26PMPuycFgyiMi_x_RN--ToheR6K6hYEGF881ALuwtNaEcntCCRM5TJvXOmejIensX7VPyItNM_bE5Limagek0YjrtMkS8milohvmMCYkAwyfEDVR3gn3pi3N_HwsMw6mPBeKPbn6t56SW8U4o4o0M9tkq18O1TPAab1_Pk7GP0CNOFQ priority: 102 providerName: ProQuest |
| Title | Detection of Nipah virus in Pteropus medius in 2019 outbreak from Ernakulam district, Kerala, India |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/33563231 https://www.proquest.com/docview/2490969773 https://www.proquest.com/docview/2488190890 https://pubmed.ncbi.nlm.nih.gov/PMC7871573 https://doaj.org/article/4902a90c419c48b188820292b0cb5ba4 |
| Volume | 21 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3db9MwELf2ISFe0PgOG5VBSDywQBJ_PyC0jk4bqGUqFFV7sWwn3apNSWkTBP895zQtixiIl6iKz41yH77fxXdnhF5IniYet4YyAw2msYtDQwUNwRuaTDjBhPLFyf0BPx7RD2M23kCr444aBi5uDO38eVKj-dXrH99-vgODf1sbvORvFrDGChX6ZIOISc5CsYm2wTMJf6JBn_7eVQBvqOpqIxGHCSF0VURz43-0HFXdz__PVfua22qnVF7zUUc76E4DLvHBUhvuoo0sv4du9Zvt8_vIvc_KOvUqx8UED6Yzc4G_T-fVAk9zfAocLmbw22-3L2-B21a4qEoIm80l9pUouAdPuKxAjXDqW-5OXbmPP_rvWmYfn-SgbA_Q6Kj35fA4bE5ZCB0ncRmSFECekVlGOIgrBQSVJinj1pGMTKIoZRPOWOqYNR7LSOGIJU5IkgFUsMoZ8hBt5UWePUY4ts5RMwEIbFMKkYq0nEsFk4WVkhoVoHjFUu2aFuT-JIwrXYcikuulGDSIQddi0CJAr9ZzZssGHP-k7npJrSl98-z6RjE_140taqqixKjI0Vg5Km0sIcqIEpXYyFl4TRqg517O2rfHyH3-zbmpFgt98umrPuCMQIRGWfw3os_DFtHLhmhSwIs609Q8ALt8260W5V6LEozctYdXOqdXNqIhcoYAFAA8CdCz9bCf6RPn8qyoPI30mE-qKECPliq6Zg4hjBPA9wESLeVtca89kk8v6hbksMzHTJAn_8OpXXQ7qe0qCSO1h7bKeZU9BSRX2g7aFGMBV3kYd9B2tzc4HXbqryKd2nDhOuye_QKDp0Sh |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtR3bbtMw1BpDAl4QdwIDDALxwKIl8SX2A0JjF610LQi2aW_GdtytGkpKk4D4Kb6R4zQpixB721sVH6fJuZ_4XBB6KXiWeL81FA44mMY2DjVNaQjWULvUpiyVvjh5NOZ7h_TDMTteQb-7WhifVtnpxEZRZ4X138g3IEwAbxu8FfJu9j30U6P86Wo3QmPBFkP36yeEbOXbwTbQ91WS7O4cbO2F7VSB0HISVyHJwKnRwjnC4fEy8BiyJGPcWOLIJIoyNuGMZZYZ7W23SC0xxKaCODCNRlpN4L5X0FVKQDR9ZfrWMqUkBmsru8IcwTdK0P2pDH0SRMQEZ2HaM37NjIB_LcE5U9hP0zxn93ZvoZutw4o3Fxx2G624_A66NmqP5O8iu-2qJp0rx8UEj6czfYp_TOd1iac5_gRUK2bw2x_hLy6BKyBxUVcQiusz7Ktb8A78w1kNrIkz38Z3aqt1PPTfyvQ6HuTAwPfQ4aXg-D5azYvcPUQ4NtZSPQG32mQUoh9hOBcSNqdGCKplgOIOpcq2bc39dI1vqglvBFcLMiggg2rIoNIAvVnumS2aelwI_d5TagnpG3I3F4r5iWrlWwF3JlpGlsbSUmFiAZFLlMjERNbAa9IAvfB0Vr7lRu5zek50XZZq8PFIbXJGIOqjLP4f0JfPPaDXLdCkgBe1uq2jAHT5Vl49yLUeJCgO21_ueE61iqtUf8UsQM-Xy36nT8bLXVF7GOH9SCGjAD1YsOgSOYQwTiBmCFDaY94e9vor-fS0aWsOpiNmKXl08WM9Q9f3Dkb7an8wHj5GN5JGupIwkmtotZrX7gn4iJV52ggmRl8vWxP8AfNydTU |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Detection+of+Nipah+virus+in+Pteropus+medius+in+2019+outbreak+from+Ernakulam+district%2C+Kerala%2C+India&rft.jtitle=BMC+infectious+diseases&rft.au=Sudeep%2C+A.+B&rft.au=Yadav%2C+Pragya+D&rft.au=Gokhale%2C+Mangesh+D&rft.au=Balasubramanian%2C+R&rft.date=2021-02-09&rft.pub=BioMed+Central+Ltd&rft.issn=1471-2334&rft.eissn=1471-2334&rft.volume=21&rft.issue=1&rft_id=info:doi/10.1186%2Fs12879-021-05865-7&rft.externalDBID=IOV&rft.externalDocID=A653576451 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1471-2334&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1471-2334&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1471-2334&client=summon |