A novel tetra-primer ARMS-PCR approach for the molecular karyotyping of chromosomal inversion 2Ru in the main malaria vectors Anopheles gambiae and Anopheles coluzzii

• Published in: Parasites & vectors Vol. 16; no. 1; p. 388
• Main Authors: Pichler, Verena, Sanou, Antoine, Love, R. Rebecca, Caputo, Beniamino, Pombi, Marco, Toe, Kobie Hyacinth, Guelbeogo, Moussa W., Sagnon, N’Fale, Ferguson, Heather M., Ranson, Hilary, Torre, Alessandra della, Besansky, Nora J.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 27.10.2023; BioMed Central; BMC
• Subjects: Adaptation; Afrotropical region; Analytical methods; Animals; Anopheles; Anopheles - genetics; Anopheles coluzzii; Anopheles gambiae; Anopheles gambiae complex; Aquatic insects; Assaying; Causes of; Chromosomal inversion; Chromosome Inversion - genetics; chromosome inversions; Chromosome rearrangements; Chromosomes; computer simulation; Constraints; cost effectiveness; Electrophoresis; Genetic aspects; Genetic polymorphisms; Genetic research; Genomes; Genotype; Genotypes; Genotyping; Human diseases; Hypotheses; Insects as carriers of disease; Inversion; Inversion (Genetics); Inversion genotyping; Inversions; Karyotyping; Life history; Malaria; Malaria vector; Methods; Molecular karyotyping; Mosquito Vectors - genetics; Mosquitoes; mutation; Nucleotide sequence; Nucleotides; Polymerase chain reaction; Polymerase Chain Reaction - methods; Polymorphism; Polymorphism, Single Nucleotide; rain; Rainfall; Sequencing; Single-nucleotide polymorphism; species; Tetra-primer ARMS-PCR; Vector-borne diseases; Vectors; Italy; United States > US; Africa; Chromosomal inversion; Inversion genotyping; Molecular karyotyping; Tetra-primer ARMS-PCR; Anopheles gambiae complex; Malaria vector

Chromosomal inversion polymorphisms have been associated with adaptive behavioral, physiological, morphological and life history traits in the two main Afrotropical malaria vectors, Anopheles coluzzii and Anopheles gambiae. The understanding of the adaptive value of chromosomal inversion systems is...