Cdc2-mediated phosphorylation of Kid controls its distribution to spindle and chromosomes

• Published in: The EMBO journal Vol. 22; no. 9; pp. 2091 - 2103
• Main Authors: Yamamoto, Tadashi, Ohsugi, Miho, Tokai-Nishizumi, Noriko, Shiroguchi, Katsuyuki, Toyoshima, Yoko Y, Inoue, Jun-ichiro
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.05.2003; Blackwell Publishing Ltd; Oxford University Press
• Subjects: ATP; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; Bacterial Toxins - chemistry; Bacterial Toxins - metabolism; Base Sequence; Cdc2; CDC2 Protein Kinase - metabolism; Chromosome Mapping; Chromosomes; Deoxyribonucleic acid; DNA; DNA Primers; HeLa Cells; Humans; Kid; kinesin-like motor; mitosis; Phosphorylation; Serine - metabolism; Threonine - metabolism

The chromokinesin Kid is important in chromosome alignment at the metaphase plate. Here, we report that Kid function is regulated by phosphorylation. We identify Ser427 and Thr463 as M phase‐specific phosphorylation sites and Cdc2–cyclin B as a Thr463 kinase. Kid with a Thr463 to alanine mutation fa...