Differential dynamics of the mammalian mRNA and protein expression response to misfolding stress

• Published in: Molecular systems biology Vol. 12; no. 1; pp. 855 - n/a
• Main Authors: Cheng, Zhe, Teo, Guoshou, Krueger, Sabrina, Rock, Tara M, Koh, Hiromi WL, Choi, Hyungwon, Vogel, Christine
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.01.2016; EMBO Press; John Wiley and Sons Inc; Springer Nature
• Subjects: Animals; Cell cycle; Central Dogma; Dithiothreitol; EMBO17; EMBO20; ER stress; Gene expression; Gene Expression Regulation - genetics; Kinetics; Mammalian cells; mammalian proteomics; Mammals; mass spectrometry; Parameter estimation; PECA; Protein Biosynthesis - genetics; Protein expression; Protein Folding; Protein Processing, Post-Translational; Protein synthesis; Proteins; Proteomics; Ratios; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Stress response; Transcription; Transcription factors; Transcription, Genetic; ER stress; mammalian proteomics; mass spectrometry; Central Dogma; PECA

The relative importance of regulation at the mRNA versus protein level is subject to ongoing debate. To address this question in a dynamic system, we mapped proteomic and transcriptomic changes in mammalian cells responding to stress induced by dithiothreitol over 30 h. Specifically, we estimated th...