Expression and Hydroxylamine Cleavage of Thymosin Alpha 1 Concatemer

• Published in: BioMed research international Vol. 2008; no. 2008; pp. 1 - 8
• Main Authors: Gong, Xing-Guo, Lu, Min-Kan, Lai, Zong-Teng, Zhou, Liang, Xie, Yi
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2008; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Amino Acid Sequence; Animals; Apoptosis; Cancer cells; Cell Line, Tumor; Cell Proliferation; Cells, Cultured; Deoxyribonucleic acid; DNA; E coli; Escherichia coli - genetics; Formazans - metabolism; Gene Expression - drug effects; Humans; Hydroxylamine - pharmacology; Inclusion Bodies - chemistry; Infections; Isopropyl Thiogalactoside - pharmacology; Life sciences; Lymphocytes - cytology; Lymphocytes - drug effects; Lymphocytes - physiology; Mice; Mice, Inbred BALB C; Mitochondria - drug effects; Mitochondria - physiology; Molecular Sequence Data; Molecular Weight; Peptide Fragments - chemistry; Peptide Fragments - genetics; Peptide Fragments - metabolism; Peptide Fragments - pharmacology; Peptides; Physiological aspects; Plasmids; Protein Structure, Secondary; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - metabolism; Tetrazolium Salts - metabolism; Thymosin; Thymosin - analogs & derivatives; Thymosin - chemical synthesis; Thymosin - chemistry; Thymosin - genetics; Thymosin - metabolism; Viral infections; China

Human thymosin alpha 1 (Tα1) is an important peptide in the development and senescence of immunological competence in human, and many studies have reported the expression of this peptide. In this study, we designed and synthesized the Tα1 gene according to the E. coli codon usage preference and cons...