influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore-derived embryos

• Published in: Protoplasma Vol. 251; no. 5; pp. 1077 - 1087
• Main Authors: Dubas, Ewa, Moravčíková, Jana, Libantová, Jana, Matušíková, Ildikó, Benková, Eva, Żur, Iwona, Krzewska, Monika
• Format: Journal Article
• Language: English
• Published: Vienna Springer-Verlag 01.09.2014; Springer Vienna; Springer Nature B.V
• Subjects: adults; Agrobacterium radiobacter; Agrobacterium tumefaciens - genetics; androgenesis; auxins; Biomedical and Life Sciences; Biosensing Techniques; biosensors; Brassica napus; Brassica napus - cytology; Brassica napus - embryology; Brassica napus - genetics; Cell Biology; cell division; Cell Division - genetics; exine; Green Fluorescent Proteins - genetics; haploidy; heat; heat stress; heat treatment; Heat-Shock Response - genetics; Hot Temperature; image analysis; in vitro culture; Indoleacetic Acids - metabolism; Life Sciences; microspores; Original; Original Article; Plant Growth Regulators - genetics; plant hormones; Plant Proteins - genetics; Plant Sciences; Plants, Genetically Modified; Pollen - cytology; Pollen - embryology; Pollen - genetics; Promoter Regions, Genetic - genetics; reporter genes; temperature; Transformation, Genetic - genetics; Zoology; DR5 promoter; Auxin; gene; Microspores; Microspore-derived embryos; Polarity; DR5rev promoter

Plant embryogenesis is regulated by differential distribution of the plant hormone auxin. However, the cells establishing these gradients during microspore embryogenesis remain to be identified. For the first time, we describe, using the DR5 or DR5rev reporter gene systems, the GFP- and GUS-based au...