WSC—DTPA纳米粒辐射防护作用的初步研究

为研究WSC．DTPA（水溶性低分子量壳聚糖WSC，二乙烯三胺五乙酸DTPA）纳米粒的辐射防护作用，采用N．乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC—DTPA纳米粒；MTT法检测其对6Gy叫Co丫射线照射后48hBRL细胞存活率的影响；活细胞工作站观察BRL细胞摄取FITC—WSC．DTPA纳米荧光探针的情况。结果表明：成功合成了游离氨基含量分别为92．7％、74.3％、1．59％的WSC—DTPA聚合物；WSC、WSC纳米粒以及WSC-DTPA纳米粒（氨基含量为92．7％，浓度在6．25μg／mL以上），随着药物浓度的增加，BRL细胞存活率均显著高于单纯照射组，差别有统计学意义（p...

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Published in辐射研究与辐射工艺学报 Vol. 30; no. 5; pp. 309 - 315
Main Author 刘蕴莹 周效旺 万建美 胡丹飞 张友九
Format Journal Article
LanguageChinese
Published 苏州大学医学部放射医学与防护学院 江苏省放射医学与防护重点实验室 苏州215123%苏州大学医学部实验中心 苏州215123 2012
Subjects
BRL细胞
二乙烯三胺五乙酸（DTPA）
水溶性低分子量壳聚糖（WSC）
纳米粒
辐射保护
纳米粒
BRL细胞
水溶性低分子量壳聚糖(WSC)
辐射保护
二乙烯三胺五乙酸(DTPA)
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ISSN1000-3436

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Summary:为研究WSC．DTPA（水溶性低分子量壳聚糖WSC，二乙烯三胺五乙酸DTPA）纳米粒的辐射防护作用，采用N．乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC—DTPA纳米粒；MTT法检测其对6Gy叫Co丫射线照射后48hBRL细胞存活率的影响；活细胞工作站观察BRL细胞摄取FITC—WSC．DTPA纳米荧光探针的情况。结果表明：成功合成了游离氨基含量分别为92．7％、74.3％、1．59％的WSC—DTPA聚合物；WSC、WSC纳米粒以及WSC-DTPA纳米粒（氨基含量为92．7％，浓度在6．25μg／mL以上），随着药物浓度的增加，BRL细胞存活率均显著高于单纯照射组，差别有统计学意义（p〈0．05），而游离氨基含量为1．59％的WSC-DTPA纳米粒无辐射保护作用；活细胞工作站检验结果显示2h内WSC纳米粒、WSC—DTPA纳米粒能够进入BRL细胞，而非纳米化的WSC-DTPA聚合物无法进入细胞。
Bibliography:Water-soluble low-molecular weight chitosan（WSC）, Diethylenetriamine pentaacetatic acid （DTPA）, Nanoparticles, BRL cells, Radiation protection.
To investigate the radiation protection of WSC-DTPA nanoparticles, N-acylation reaction and ion crossing-linking method were used to prepare nanoparticles in different remaining free amino groups. MTT assay was performed to evaluate their effects on the viability of the cells after y-rays irradiation, while the capability of FITC-WSC-DTPA nanoparticles probe penetrating through cellular membrane was observed by Live Cell Station. The results show that WSC-DTPA polymers were successfully synthesized, in which the content free amino group is 92.7%, 74.3%, 1.59%, respectively. Compared with y-rays alone group, it was shown that WSC, WSC nanoparticles and WSC-DTPA nanoparticles with the concentration more than 6.25 μg/mL and the free amino group content around 92.7% have good efficacy in radiation protection to BRL cells from damage induced by 6 Gy 60Co y-rays by the highe
ISSN:1000-3436