气相色谱-质谱法快速测定鱼油中的生育酚
目的建立气相色谱-质谱法（gas chromatography-mass spectrometry,GC-MS）测定鱼油中生育酚含量的分析方法。方法样品经正己烷提取,以HP-5MS毛细管柱分离,GC-MS选择离子检测模式测定。结果α-、γ-和δ-生育酚在0.002-0.04 mg/mL的浓度范围内呈良好线性关系,相关系数r^2〉0.999。此方法α-、γ-和δ-生育酚的检出限（S/N=3）分别为0.001、0.002、0.001 mg/g,定量限（S/N=10）为0.003、0.006、0.004 mg/g。在不同添加水平下,方法的平均回收率分别为98.6%-99.9%,相对标准偏差小于1.0...
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| Published in | 食品安全质量检测学报 Vol. 8; no. 9; pp. 3390 - 3395 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
汤臣倍健股份有限公司,珠海,519040
2017
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| Subjects | |
| Online Access | Get full text |
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| Summary: | 目的建立气相色谱-质谱法（gas chromatography-mass spectrometry,GC-MS）测定鱼油中生育酚含量的分析方法。方法样品经正己烷提取,以HP-5MS毛细管柱分离,GC-MS选择离子检测模式测定。结果α-、γ-和δ-生育酚在0.002-0.04 mg/mL的浓度范围内呈良好线性关系,相关系数r^2〉0.999。此方法α-、γ-和δ-生育酚的检出限（S/N=3）分别为0.001、0.002、0.001 mg/g,定量限（S/N=10）为0.003、0.006、0.004 mg/g。在不同添加水平下,方法的平均回收率分别为98.6%-99.9%,相对标准偏差小于1.0%（n=3）。结论该方法简便、快速、灵敏、准确,适合鱼油中生育酚的定性和定量测定。 |
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| Bibliography: | tocopherol; gas chromatography-mass spectrometry; fish oil Objective To establish a method for rapid determination of tocopherol in fish oil by gas chromatography-mass spectrometry （GC-MS）. Methods The samples were extracted by n-hexane, and separated by HP-5MS capillary column. Then they were detected by GC-MS with selected ion monitoring mode. Results The α-, γ-,δ-tocopherol had good linear relationships in the range of 0.002-0.04 mg/mL, and the correlation coefficients （r2） were over 0.999. The limits of detection （S/N=3） were 0.001, 0.002, 0.001 mg/g, and the limits of quantification （S/N=10） were 0.003, 0.006, 0.004 mg/g, respectively. The average recoveries at 3 different spiked levels were 98.6%-99.9%, and the relative standard deviations were less than 1.0% （n=3）. Conclusion The established method is simple, rapid, sensitive and accurate, which is suitable for the qualitative and quantitative determination of tocopherol in fish oil. 11-5956/TS ZHANG Xi-Jin, WEI Xian-E, CAI Wei-Jiang, CHEN Cai-Yun, LI Xia |
| ISSN: | 2095-0381 |