Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass

• Published in: Biotechnology for biofuels Vol. 14; no. 1; p. 101
• Main Authors: Kirby, James, Geiselman, Gina M., Yaegashi, Junko, Kim, Joonhoon, Zhuang, Xun, Tran-Gyamfi, Mary Bao, Prahl, Jan-Philip, Sundstrom, Eric R., Gao, Yuqian, Munoz, Nathalie, Burnum-Johnson, Kristin E., Benites, Veronica T., Baidoo, Edward E. K., Fuhrmann, Anna, Seibel, Katharina, Webb-Robertson, Bobbie-Jo M., Zucker, Jeremy, Nicora, Carrie D., Tanjore, Deepti, Magnuson, Jon K., Skerker, Jeffrey M., Gladden, John M.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 21.04.2021; BioMed Central; BMC
• Subjects: 09 BIOMASS FUELS; 1,8-Cineole; BASIC BIOLOGICAL SCIENCES; Basidiomycota; Biodiesel fuels; Biofuels; Biomass; Biosynthesis; biotechnology; Bisabolene; Carbon; Cineole; Climate change; Climate change mitigation; Coenzyme A; Copy number; Engineering; Eucalyptol; feedstocks; fermenters; Gene expression; Genes; Genetic aspects; Hydrolysates; Hydroxymethylglutaryl-CoA reductase; Industrial microorganisms; Intermediates; Isoprenoids; Kinases; Lignocellulose; Metabolic engineering; Metabolism; Metabolites; Methods; Mevalonate kinase; Mevalonate pathway; Mevalonic acid; Microbiological synthesis; Microorganisms; Nitrogen; Optimization; Phosphomevalonate kinase; Physiological aspects; Production processes; Proteins; Reductases; Rhodosporidium toruloides; Rhodotorula; sesquiterpenoids; Terpenes; Α-bisabolene; United States; Eucalyptol; Α-bisabolene; 1,8-Cineole; Mevalonate pathway; Rhodotorula; Metabolic engineering; Isoprenoids

Mitigation of climate change requires that new routes for the production of fuels and chemicals be as oil-independent as possible. The microbial conversion of lignocellulosic feedstocks into terpene-based biofuels and bioproducts represents one such route. This work builds upon previous demonstratio...