silencing suppressor P25 of Potato virus X interacts with Argonaute1 and mediates its degradation through the proteasome pathway

• Published in: Molecular plant pathology Vol. 11; no. 5; pp. 641 - 649
• Main Authors: CHIU, MENG-HSUEN, CHEN, I-HSUAN, BAULCOMBE, DAVID C, TSAI, CHING-HSIU
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.09.2010; Blackwell Publishing Ltd; Blackwell
• Subjects: Arabidopsis; Argonaute 2 protein; Bamboo mosaic virus; Biological and medical sciences; defense mechanisms; drug effects; Fundamental and applied biological sciences. Psychology; Gene Silencing; Gene Silencing - drug effects; Genes, Suppressor; genetics; Leupeptins; Leupeptins - pharmacology; metabolism; Nicotiana - drug effects; Nicotiana - metabolism; Nicotiana - virology; Nicotiana benthamiana; Nuclease; Original; p25 protein; pharmacology; Phytopathology. Animal pests. Plant and forest protection; Plant Proteins; Plant Proteins - metabolism; Plant viruses; potato protein; Potato virus X; Potexvirus; Potexvirus - drug effects; Potexvirus - metabolism; proteasome endopeptidase complex; Proteasome Endopeptidase Complex - metabolism; Proteasome Inhibitors; proteasomes; Protein Binding; Protein Binding - drug effects; protein degradation; Protein Processing, Post-Translational; Protein Processing, Post-Translational - drug effects; Protein Stability; Protein Stability - drug effects; Protoplasts; Protoplasts - drug effects; Protoplasts - metabolism; Protoplasts - virology; RNA; RNA-mediated interference; Signal Transduction; Signal Transduction - drug effects; Solanum tuberosum; Tobacco; Viral Proteins; Viral Proteins - genetics; Viral Proteins - metabolism; virology; Western blotting; Virus; Degradation; Peptidases; Multicatalytic endopeptidase complex; Plant pathogen; Potato virus X; Enzyme; Flexiviridae; Potexvirus; Hydrolases; Suppressor

Previous evidence has indicated that the P25 protein encoded by Potato virus X (PVX) inhibits either the assembly or function of the effector complexes in the RNA silencing-based antiviral defence system (Bayne et al., Cell-to-cell movement of Potato Potexvirus X is dependent on suppression of RNA s...