Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains

• Published in: BMC genomics Vol. 18; no. 1; pp. 451 - 14
• Main Authors: Silva, Wanderson M., Folador, Edson L., Soares, Siomar C., Souza, Gustavo H. M. F., Santos, Agenor V., Sousa, Cassiana S., Figueiredo, Henrique, Miyoshi, Anderson, Le Loir, Yves, Silva, Artur, Azevedo, Vasco
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.06.2017; BioMed Central; BMC
• Subjects: Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; Caseous lymphadenitis; Chromatography; Confidence; Corynebacterium pseudotuberculosis; Corynebacterium pseudotuberculosis - classification; Corynebacterium pseudotuberculosis - genetics; Corynebacterium pseudotuberculosis - isolation & purification; Corynebacterium pseudotuberculosis - metabolism; Etiology; Food and Nutrition; Food engineering; Gene sequencing; Genomes; Genomics; Informatics; Information processing; Information storage; Ions; Label-free proteomics, proteomic; Life Sciences; Lymphadenitis; Lymphangitis; Mass spectrometry; Metabolic Networks and Pathways; Microbiology and Parasitology; Pathogens; Peptides; Physiology; Proteins; Proteome; Proteomes; Proteomic bacterial; Proteomics; Pseudotuberculosis; Scientific imaging; Sheep; Signal Transduction; Statistical analysis; Ulcerative lymphangitis; Virulence; Belgium; Brazil; United States > US; Corynebacterium pseudotuberculosis; Ulcerative lymphangitis; Caseous lymphadenitis; Label-free proteomics, proteomic; Proteomic bacterial; caractérisation souche bactérienne; protéomique; animal health; approche protéomique; souche bactérienne; corynebacterium pseudotuberculosis; santé animale; séquence génomique
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-017-3835-y

Background Corynebacterium pseudotuberculosis is a pathogen classified into two biovars: C. pseudotuberculosis biovar ovis, the etiologic agent of caseous lymphadenitis and C. pseudotuberculosis biovar equi, which causes ulcerative lymphangitis. The available whole genome sequences of different C. pseudotuberculosis strains have enabled identify difference of genes related both virulence and physiology of each biovar. To evaluate be this difference could reflect at proteomic level and to better understand the shared factors and the exclusive ones of biovar ovis and biovar equi strains, we applied the label-free quantitative proteomic to characterize the proteome of the strains: 1002_ovis and 258_equi, isolated from goat (Brazil) and equine (Belgium), respectively. Results From this analysis, we characterized a total of 1230 proteins in 1002_ovis and 1220 in 258_equi with high confidence. Moreover, the core-proteome between 1002_ovis and 258_equi obtained here is composed of 1122 proteins involved in different cellular processes, which could be necessary for the free living of C. pseudotuberculosis. In addition, 120 proteins from this core-proteome presented change in abundant with statistically significant differences. Considering the exclusive proteome, we detected strain-specific proteins to each strain. When correlated, the exclusive proteome of each strain and proteome with change in abundant, the proteomic differences, between the 1002_ovis and 258_equi, this related to proteins involved in cellular metabolism, information storage and processing, cellular processes and signaling. Conclusions This study reports the first comparative proteomic study of the biovars ovis and equi of C. pseudotuberculosis. The results generated in this study provide information about factors which can contribute to understanding both the physiology and the virulence of this pathogen.