Global Patterns of Tissue-Specific Alternative Polyadenylation in Drosophila

• Published in: Cell reports (Cambridge) Vol. 1; no. 3; pp. 277 - 289
• Main Authors: Smibert, Peter, Miura, Pedro, Westholm, Jakub O., Shenker, Sol, May, Gemma, Duff, Michael O., Zhang, Dayu, Eads, Brian D., Carlson, Joe, Brown, James B., Eisman, Robert C., Andrews, Justen, Kaufman, Thomas, Cherbas, Peter, Celniker, Susan E., Graveley, Brenton R., Lai, Eric C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 29.03.2012; Elsevier
• Subjects: 3' Untranslated Regions - genetics; Animals; Base Sequence; Blotting, Northern; Conserved Sequence - genetics; DNA-Binding Proteins - metabolism; Drosophila melanogaster; Drosophila melanogaster - embryology; Drosophila melanogaster - genetics; Embryo, Nonmammalian - metabolism; Gene Expression Regulation, Developmental; Genes, Insect - genetics; In Situ Hybridization; Male; Molecular Sequence Data; Neurons - cytology; Neurons - metabolism; Nucleotide Motifs - genetics; Organ Specificity - genetics; Poly A - metabolism; Polyadenylation - genetics; Protein Isoforms - genetics; Protein Isoforms - metabolism; Reproducibility of Results; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Analysis, RNA; Testis - metabolism; Transcriptome - genetics

We analyzed the usage and consequences of alternative cleavage and polyadenylation (APA) in Drosophila melanogaster by using >1 billion reads of stranded mRNA-seq across a variety of dissected tissues. Beyond demonstrating that a majority of fly transcripts are subject to APA, we observed broad t...