Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

• Published in: Microbial cell factories Vol. 16; no. 1; p. 18
• Main Authors: Beneyton, Thomas, Thomas, Stéphane, Griffiths, Andrew D., Nicaud, Jean-Marc, Drevelle, Antoine, Rossignol, Tristan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 31.01.2017; BioMed Central
• Subjects: Aspartic Acid Proteases - genetics; Aspartic Acid Proteases - metabolism; Aspergillus niger - genetics; Biocatalysis; Cellulase - genetics; Cellulase - metabolism; Cellulose 1,4-beta-Cellobiosidase - genetics; Cellulose 1,4-beta-Cellobiosidase - metabolism; Condensed Matter; E coli; Endo-1,4-beta Xylanases - genetics; Endo-1,4-beta Xylanases - metabolism; Enzymatic activity; Escherichia coli; Gene Expression; High-Throughput Screening Assays - methods; Hydrolysis; Microfluidics - methods; Physics; Physiological aspects; Proteases; Protein Engineering - methods; Recombinant proteins; Recombinant Proteins - metabolism; Yarrowia - enzymology; Yarrowia - genetics; Yarrowia - metabolism; Yeasts; France; High-throughput enzymatic screening; Recombinant fungal protein; Droplet-based microfluidics; Heterologous expression; Yarrowia lipolytica
• ISSN: 1475-2859; 1475-2859
• DOI: 10.1186/s12934-017-0629-5

Droplet-based microfluidics is becoming an increasingly attractive alternative to microtiter plate techniques for enzymatic high-throughput screening (HTS), especially for exploring large diversities with lower time and cost footprint. In this case, the assayed enzyme has to be accessible to the sub...