Regenerating 1 and 3b gene expression in the pancreas of type 2 diabetic Goto-Kakizaki (GK) rats

• Published in: PloS one Vol. 9; no. 2; p. e90045
• Main Authors: Calderari, Sophie, Irminger, Jean-Claude, Giroix, Marie-Hélène, Ehses, Jan A, Gangnerau, Marie-Noëlle, Coulaud, Josiane, Rickenbach, Katharina, Gauguier, Dominique, Halban, Philippe, Serradas, Patricia, Homo-Delarche, Françoise
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.02.2014; Public Library of Science (PLoS)
• Subjects: Acinar cells; Angiogenesis; Animals; Antibodies; Antigens, Neoplasm - genetics; Antigens, Neoplasm - metabolism; Biology; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Cell culture; Cell growth; Cell survival; Chemokines; Chemokines - blood; Chemokines - secretion; Cytokines; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - genetics; Diabetes Mellitus, Type 2 - metabolism; Diabetes Mellitus, Type 2 - pathology; Experiments; Gene expression; Gene Expression Regulation; Genes; Homeostasis; Human health and pathology; Hyperglycemia; Immunohistochemistry; Infiltration; Inflammation; Insulin; Interleukin 6; Interleukins; Islets of Langerhans; Islets of Langerhans - metabolism; Lectins, C-Type - genetics; Lectins, C-Type - metabolism; Life Sciences; Ligands; Lithostathine - genetics; Lithostathine - metabolism; Localization; Macrophages; Macrophages - metabolism; Major histocompatibility complex; Male; Medical research; Medicine; MHC antibodies; Pancreas; Pancreatitis; Pancreatitis-Associated Proteins; Paraffin; Polymerase chain reaction; Proteins; Rats; Rats, Wistar; RNA; Rodents; Tumor necrosis factor-TNF; Type 2 diabetes; Vascular endothelial growth factor; France
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0090045

Regenerating (REG) proteins are associated with islet development, β-cell damage, diabetes and pancreatitis. Particularly, REG-1 and REG-3-beta are involved in cell growth/survival and/or inflammation and the Reg1 promoter contains interleukin-6 (IL-6)-responsive elements. We showed by transcriptome analysis that islets of Goto-Kakizaki (GK) rats, a model of spontaneous type 2 diabetes, overexpress Reg1, 3α, 3β and 3γ, vs Wistar islets. Goto-Kakizaki rat islets also exhibit increased cytokine/chemokine expression/release, particularly IL-6. Here we analyzed Reg1 and Reg3β expression and REG-1 immuno-localization in the GK rat pancreas in relationship with inflammation. Isolated pancreatic islets and acinar tissue from male adult Wistar and diabetic GK rats were used for quantitative RT-PCR analysis. REG-1 immunohistochemistry was performed on paraffin sections with a monoclonal anti-rat REG-1 antibody. Islet cytokine/chemokine release was measured after 48 h-culture. Islet macrophage-positive area was quantified on cryostat sections using anti-CD68 and major histocompatibility complex (MHC) class II antibodies. Pancreatic exocrine-to-endocrine Reg1 and Reg3β mRNA ratios were markedly increased in Wistar vs GK rats. Conversely, both genes were upregulated in isolated GK rat islets. These findings were unexpected, because Reg genes are expressed in the pancreatic acinar tissue. However, we observed REG-1 protein labeling in acinar peri-ductal tissue close to islets and around large, often disorganized, GK rat islets, which may retain acinar cells due to their irregular shape. These large islets also showed peri-islet macrophage infiltration and increased release of various cytokines/chemokines, particularly IL-6. Thus, IL-6 might potentially trigger acinar REG-1 expression and secretion in the vicinity of large diabetic GK rat islets. This increased acinar REG-1 expression might reflect an adaptive though unsuccessful response to deleterious microenvironment.