Hypophosphatasia-associated Deficiencies in Mineralization and Gene Expression in Cultured Dental Pulp Cells Obtained from Human Teeth

• Published in: Journal of endodontics Vol. 38; no. 7; pp. 907 - 912
• Main Authors: Rodrigues, Thaisângela L., Foster, Brian L., Silverio, Karina G., Martins, Luciane, Casati, Marcio Z., Sallum, Enilson A., Somerman, Martha J., Nociti, Francisco H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2012
• Subjects: Adolescent; Alkaline phosphatase; Alkaline Phosphatase - genetics; Amino Acid Substitution; Analysis of Variance; Calcium - metabolism; Case-Control Studies; Dental Pulp - cytology; Dental Pulp - physiopathology; dental pulp cells; dentin; Dentin - pathology; Dentistry; Diphosphates - metabolism; Diseases in Twins - genetics; Down-Regulation; Endocrinology & Metabolism; Extracellular Matrix Proteins - biosynthesis; Extracellular Matrix Proteins - genetics; Female; Gene Expression; Glycoproteins - biosynthesis; Glycoproteins - genetics; Humans; hypophosphatasia; Hypophosphatasia - genetics; Hypophosphatasia - pathology; Hypophosphatasia - physiopathology; Male; Mutation, Missense; Odontoblasts - metabolism; Odontoblasts - pathology; Osteopontin - biosynthesis; Osteopontin - genetics; Phosphate Transport Proteins - biosynthesis; Phosphate Transport Proteins - genetics; Phosphoproteins - biosynthesis; Phosphoproteins - genetics; Phosphoric Diester Hydrolases - biosynthesis; Phosphoric Diester Hydrolases - genetics; Primary Cell Culture; Pyrophosphatases - biosynthesis; Pyrophosphatases - genetics; pyrophosphate; Sialoglycoproteins - biosynthesis; Sialoglycoproteins - genetics; Statistics, Nonparametric; Tooth Calcification - genetics; Young Adult; pyrophosphate; Alkaline phosphatase; hypophosphatasia; dental pulp cells; dentin
• ISSN: 0099-2399; 1878-3554; 1878-3554
• DOI: 10.1016/j.joen.2012.02.008

Mutations in the gene ALPL in hypophosphatasia (HPP) reduce the function of tissue nonspecific alkaline phosphatase, and the resulting increase in pyrophosphate (PPi) contributes to bone and tooth mineralization defects by inhibiting physiologic calcium-phosphate (Pi) precipitation. Although periodo...