Juvenile social defeat stress exposure favors in later onset of irritable bowel syndrome-like symptoms in male mice

• Published in: Scientific reports Vol. 11; no. 1; p. 16276
• Main Authors: Matsumoto, Kenjiro, Takata, Kana, Yamada, Daisuke, Usuda, Haruki, Wada, Koichiro, Tada, Maaya, Mishima, Yoshiyuki, Ishihara, Shunji, Horie, Syunji, Saitoh, Akiyoshi, Kato, Shinichi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11.08.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/378; 692/4020; Amygdala; Avoidance behavior; Calcitonin; Calcitonin gene-related peptide; Colon; Corticosterone; Humanities and Social Sciences; Hypersensitivity; Immune response; Intestinal microflora; Intestine; Irritable bowel syndrome; Metabolites; Microbiota; multidisciplinary; Norepinephrine; Science; Science (multidisciplinary); Social interactions
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-95916-5

Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder. Traumatic stress during adolescence increases the risk of IBS in adults. The aim of this study was to characterize the juvenile social defeat stress (SDS)-associated IBS model in mice. Juvenile mice were exposed to an aggressor mouse for 10 min once daily for 10 consecutive days. Behavioral tests, visceral sensitivity, immune responses, and fecal bacteria in the colon were evaluated in 5 weeks after SDS exposure. Social avoidance, anxiety- and depression-like behavior, and visceral hypersensitivity were observed. Juvenile SDS exposure significantly increased the number of 5-HT-containing cells and calcitonin gene-related peptide-positive neurons in the colon. The gut microbiota was largely similar between the control and juvenile SDS groups. The alterations in fecal pellet output, bead expulsion time, plasma corticosterone concentration, and colonic 5-HT content in response to restraint stress were exacerbated in the juvenile SDS group compared with the control group. The combination of juvenile SDS and restraint stress increased the noradrenaline metabolite 3-Methoxy-4-hydroxyphenylglycol (MHPG) content and MHPG/noradrenaline ratio in the amygdala when compared with restraint stress in control mice. These results suggest that juvenile SDS exposure results in later onset of IBS-like symptoms.