Ookinete Antigens of Plasmodium berghei: A Light and Electron-Microscope Immunogold Study of Expression of the 21 kDa Determinant Recognized by a Transmission-Blocking Antibody
The expression of a 21 kDa transmission-blocking determinant on the malarial parasite Plasmodium berghei was studied by using the immunogold method at the light, scanning-electron and transmission-electron microscope levels. The determinant was shown to be expressed exclusively on the macrogamete an...
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Published in | Proceedings of the Royal Society of London. Series B, Containing papers of a biological character Vol. 230; no. 1261; p. 443 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
The Royal Society
22.05.1987
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Subjects | |
Online Access | Get full text |
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Summary: | The expression of a 21 kDa transmission-blocking determinant on the malarial parasite Plasmodium berghei was studied by using
the immunogold method at the light, scanning-electron and transmission-electron microscope levels. The determinant was shown
to be expressed exclusively on the macrogamete and its immediate progeny the zygote, ookinete and oocyst. It is first detected
on the plasmalemma two hours after the escape of the parasite from the red blood cell, reaches a maximal density on the young
ookinete some ten hours later, and is still found on the oocyst after six days. The antigen is distributed evenly over the
entire surface of the zygote and ookinete, but is readily shed from the parasite surface. The general applicability of the
silver-enhanced immunogold method in parasitological research is emphasized. |
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ISSN: | 0962-8452 0950-1193 1471-2954 |
DOI: | 10.1098/rspb.1987.0028 |