Epidermal Growth Factor Receptor Dimerization and Activation Require Ligand-Induced Conformational Changes in the Dimer Interface

Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue MCB About MCB Subscribers Authors Reviewers Advertisers Inquiries from...

Full description

Saved in:
Bibliographic Details
Published inMolecular and Cellular Biology Vol. 25; no. 17; pp. 7734 - 7742
Main Authors Dawson, Jessica P., Berger, Mitchell B., Lin, Chun-Chi, Schlessinger, Joseph, Lemmon, Mark A., Ferguson, Kathryn M.
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.09.2005
Taylor & Francis
Subjects
Binding Sites
Dimerization
Enzyme Activation
Ligands
Models, Molecular
Molecular Sequence Data
Mutation - genetics
Protein Structure, Quaternary
Protein Structure, Tertiary
Receptor, Epidermal Growth Factor - chemistry
Receptor, Epidermal Growth Factor - genetics
Receptor, Epidermal Growth Factor - metabolism
Signal Transduction
Surface Plasmon Resonance
Transforming Growth Factor alpha - pharmacology
Online AccessGet full text

Cover

Abstract Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue MCB About MCB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy MCB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0270-7306 Online ISSN: 1098-5549 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to MCB .asm.org, visit: MCB       
AbstractList Structural studies have shown that ligand-induced epidermal growth factor receptor (EGFR) dimerization involves major domain rearrangements that expose a critical dimerization arm. However, simply exposing this arm is not sufficient for receptor dimerization, suggesting that additional ligand-induced dimer contacts are required. To map these contributions to the dimer interface, we individually mutated each contact suggested by crystallographic studies and analyzed the effects on receptor dimerization, activation, and ligand binding. We find that domain II contributes >90% of the driving energy for dimerization of the extracellular region, with domain IV adding little. Within domain II, the dimerization arm forms much of the dimer interface, as expected. However, a loop from the sixth disulfide-bonded module (immediately C-terminal to the dimerization arm) also makes a critical contribution. Specific ligand-induced conformational changes in domain II are required for this loop to contribute to receptor dimerization, and we identify a set of ligand-induced intramolecular interactions that appear to be important in driving these changes, effectively "buttressing" the dimer interface. Our data also suggest that similar conformational changes may determine the specificity of ErbB receptor homo- versus heterodimerization.
Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue MCB About MCB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy MCB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0270-7306 Online ISSN: 1098-5549 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to MCB .asm.org, visit: MCB       
Structural studies have shown that ligand-induced epidermal growth factor receptor (EGFR) dimerization involves major domain rearrangements that expose a critical dimerization arm. However, simply exposing this arm is not sufficient for receptor dimerization, suggesting that additional ligand-induced dimer contacts are required. To map these contributions to the dimer interface, we individually mutated each contact suggested by crystallographic studies and analyzed the effects on receptor dimerization, activation, and ligand binding. We find that domain II contributes >90% of the driving energy for dimerization of the extracellular region, with domain IV adding little. Within domain II, the dimerization arm forms much of the dimer interface, as expected. However, a loop from the sixth disulfide-bonded module (immediately C-terminal to the dimerization arm) also makes a critical contribution. Specific ligand-induced conformational changes in domain II are required for this loop to contribute to receptor dimerization, and we identify a set of ligand-induced intramolecular interactions that appear to be important in driving these changes, effectively "buttressing" the dimer interface. Our data also suggest that similar conformational changes may determine the specificity of ErbB receptor homo- versus heterodimerization.
Author Jessica P. Dawson
Chun-Chi Lin
Mitchell B. Berger
Mark A. Lemmon
Kathryn M. Ferguson
Joseph Schlessinger
AuthorAffiliation Department of Biochemistry and Biophysics, 1 Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 2 Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 3
AuthorAffiliation_xml – name: Department of Biochemistry and Biophysics, 1 Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 2 Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 3
Author_xml – sequence: 1
  givenname: Jessica P.
  surname: Dawson
  fullname: Dawson, Jessica P.
  organization: Department of Biochemistry and Biophysics
– sequence: 2
  givenname: Mitchell B.
  surname: Berger
  fullname: Berger, Mitchell B.
  organization: Department of Biochemistry and Biophysics
– sequence: 3
  givenname: Chun-Chi
  surname: Lin
  fullname: Lin, Chun-Chi
  organization: Department of Physiology, University of Pennsylvania School of Medicine
– sequence: 4
  givenname: Joseph
  surname: Schlessinger
  fullname: Schlessinger, Joseph
  organization: Department of Pharmacology, Yale University School of Medicine
– sequence: 5
  givenname: Mark A.
  surname: Lemmon
  fullname: Lemmon, Mark A.
  email: mlemmon@mail.med.upenn.edu
  organization: Department of Biochemistry and Biophysics
– sequence: 6
  givenname: Kathryn M.
  surname: Ferguson
  fullname: Ferguson, Kathryn M.
  organization: Department of Physiology, University of Pennsylvania School of Medicine
BackLink https://www.ncbi.nlm.nih.gov/pubmed/16107719$$D View this record in MEDLINE/PubMed
BookMark eNqNkU1v1DAQhi1URLeFv4AsDtwSbCeO4wtSST9YaRFSBWfLcSYbo8Te2klX5cY_J1FWUG6cbGveecaa5wKdOe8AIUxJSikrP3ypPqWMp1SkQmR5IkTOUkYIf4E2lMgy4TyXZ2hDmCCJyEhxji5i_EEIKSTJXqFzWlAiBJUb9OvmYBsIg-7xXfDHscO32ow-4HswcFgu13aAYH_q0XqHtWvwlRnt4_q8h4fJBsA7u58rydY1k4EGV961fmYumRlcddrtIWLr8NjBCsRbN0JotYHX6GWr-whvTucl-n578636nOy-3m2rq11iClaMSVEDkYxwo3Nd87IBVkva8EY2BKTUbSa4AWaELmVtCGQkbzXRLamNzNpCsuwSfVy5h6keoDHgxqB7dQh20OFJeW3VvxVnO7X3j4pSOS8ymwHvT4DgHyaIoxpsNND32oGfoirKXHLCyzlYrkETfIwB2j9DKFGLPzX7U4wrKtTiTy3-1OJvbn37_JN_G0_C5kC1Buy64qMPfaNG_dT70AbtjI0q-48x71ZKZ_fdcTaodBzUYOpn8ew3ApO9rw
CitedBy_id crossref_primary_10_1016_j_biotechadv_2014_03_004
crossref_primary_10_1016_j_str_2007_11_009
crossref_primary_10_1371_journal_pcbi_1003201
crossref_primary_10_3390_biomedicines9010039
crossref_primary_10_1073_pnas_1507329112
crossref_primary_10_1016_j_ijbiomac_2020_07_290
crossref_primary_10_1038_onc_2016_393
crossref_primary_10_1186_bcr1633
crossref_primary_10_1073_pnas_0507591102
crossref_primary_10_1016_j_jim_2023_113456
crossref_primary_10_1016_j_wndm_2016_02_002
crossref_primary_10_1242_jcs_033399
crossref_primary_10_1101_cshperspect_a041419
crossref_primary_10_18632_oncotarget_27730
crossref_primary_10_1021_bi401632z
crossref_primary_10_1371_journal_pone_0069325
crossref_primary_10_3390_biotech12030057
crossref_primary_10_1021_acs_biochem_0c00641
crossref_primary_10_2217_bmm_09_9
crossref_primary_10_1080_07391102_2015_1095114
crossref_primary_10_3390_md20100596
crossref_primary_10_3390_epigenomes7040024
crossref_primary_10_1083_jcb_201711175
crossref_primary_10_1111_j_1747_0285_2010_00986_x
crossref_primary_10_1002_btpr_185
crossref_primary_10_1002_cam4_1236
crossref_primary_10_1016_j_drudis_2016_10_004
crossref_primary_10_1074_jbc_M110_182899
crossref_primary_10_1126_sciadv_adj7251
crossref_primary_10_3892_mmr_2019_10259
crossref_primary_10_3389_fcell_2021_732952
crossref_primary_10_1073_pnas_1105666108
crossref_primary_10_1371_journal_pgen_1000177
crossref_primary_10_1002_ijc_30649
crossref_primary_10_3389_fphar_2023_1090500
crossref_primary_10_1073_pnas_1107385108
crossref_primary_10_1097_CAD_0000000000000510
crossref_primary_10_1002_ijch_202300163
crossref_primary_10_1007_s00894_015_2580_x
crossref_primary_10_1016_j_bmcl_2018_10_005
crossref_primary_10_2174_0929867328666210810154732
crossref_primary_10_2217_fon_14_198
crossref_primary_10_1158_0008_5472_CAN_13_1145
crossref_primary_10_1016_j_cellsig_2005_12_007
crossref_primary_10_1002_ange_201201805
crossref_primary_10_1158_1541_7786_MCR_15_0240
crossref_primary_10_3892_or_2013_2754
crossref_primary_10_1021_acschembio_6b00990
crossref_primary_10_1038_srep10300
crossref_primary_10_1073_pnas_1201114109
crossref_primary_10_3889_oamjms_2022_9209
crossref_primary_10_18410_jebmh_2019_594
crossref_primary_10_1016_j_str_2007_06_013
crossref_primary_10_1038_nature08297
crossref_primary_10_1021_acs_analchem_7b03448
crossref_primary_10_1021_bi400437d
crossref_primary_10_1016_j_jhep_2007_08_009
crossref_primary_10_1517_14656566_7_10_1251
crossref_primary_10_1002_jmr_808
crossref_primary_10_1038_s41388_018_0537_0
crossref_primary_10_1128_JVI_02095_17
crossref_primary_10_1016_j_brainresbull_2010_06_008
crossref_primary_10_1007_s10930_021_10021_w
crossref_primary_10_1111_j_1365_2559_2010_03494_x
crossref_primary_10_1021_bi101843s
crossref_primary_10_4236_jct_2014_56055
crossref_primary_10_1073_pnas_0601469103
crossref_primary_10_1158_1078_0432_CCR_12_0989
crossref_primary_10_1016_j_bmc_2015_03_040
crossref_primary_10_1371_journal_ppat_1003325
crossref_primary_10_1080_17425247_2021_1825377
crossref_primary_10_1248_cpb_c18_00539
crossref_primary_10_1073_pnas_0915146107
crossref_primary_10_1016_j_biochi_2021_07_002
crossref_primary_10_1021_acs_chemrev_1c01035
crossref_primary_10_1002_prot_24257
crossref_primary_10_1007_s00249_008_0293_0
crossref_primary_10_3109_08977194_2012_739619
crossref_primary_10_1016_j_sbi_2014_10_002
crossref_primary_10_1021_acsomega_7b00934
crossref_primary_10_1016_j_bbrc_2007_10_004
crossref_primary_10_7554_eLife_14107
crossref_primary_10_1089_ars_2013_5469
crossref_primary_10_1007_s11523_016_0456_3
crossref_primary_10_1038_onc_2010_313
crossref_primary_10_5005_jp_journals_10001_1044
crossref_primary_10_1016_j_ccr_2013_04_012
crossref_primary_10_1074_jbc_M112_414391
crossref_primary_10_1371_journal_pone_0139971
crossref_primary_10_1038_s41421_023_00523_5
crossref_primary_10_1124_pharmrev_121_000381
crossref_primary_10_1515_hsz_2016_0129
crossref_primary_10_3390_s18030781
crossref_primary_10_1016_j_bbamcr_2006_05_002
crossref_primary_10_1016_j_yexcr_2018_08_009
crossref_primary_10_18632_oncotarget_16300
crossref_primary_10_1002_bmb_20211
crossref_primary_10_1038_srep33507
crossref_primary_10_1016_j_bbamem_2017_03_026
crossref_primary_10_1016_j_xphs_2020_10_015
crossref_primary_10_1039_c2mb25190f
crossref_primary_10_1371_journal_pone_0177761
crossref_primary_10_3390_ijms241311110
crossref_primary_10_1038_s41586_021_04393_3
crossref_primary_10_3233_CBM_170466
crossref_primary_10_1371_journal_pone_0118796
crossref_primary_10_1007_s10637_023_01373_4
crossref_primary_10_1038_ncomms8380
crossref_primary_10_47836_mjmhs_20_1_37
crossref_primary_10_1016_j_bioorg_2018_07_015
crossref_primary_10_1016_j_ijbiomac_2018_04_066
crossref_primary_10_1038_nsmb_2135
crossref_primary_10_1111_cas_14872
crossref_primary_10_1007_s12031_008_9060_z
crossref_primary_10_1371_journal_pcbi_1005222
crossref_primary_10_3390_vaccines10040499
crossref_primary_10_1210_me_2005_0291
crossref_primary_10_1126_scisignal_287re6
crossref_primary_10_1016_j_pharmthera_2008_11_008
crossref_primary_10_1042_BJ20071163
crossref_primary_10_1016_j_celrep_2014_10_010
crossref_primary_10_1073_pnas_1803152115
crossref_primary_10_1002_anie_201201805
crossref_primary_10_1530_ERC_19_0009
crossref_primary_10_1007_s13277_015_4742_y
crossref_primary_10_1186_s12885_016_2796_x
crossref_primary_10_1016_j_csbj_2020_03_005
crossref_primary_10_3389_fphar_2021_626233
crossref_primary_10_1016_j_cellsig_2018_07_010
crossref_primary_10_7554_eLife_00708
crossref_primary_10_1016_j_yexcr_2008_10_008
crossref_primary_10_1007_s10930_020_09943_8
crossref_primary_10_1074_jbc_M704592200
crossref_primary_10_12677_biphy_2013_11002
crossref_primary_10_4161_cam_4_2_11191
crossref_primary_10_1248_yakushi_14_00240_1
crossref_primary_10_18632_oncotarget_11582
crossref_primary_10_1038_cdd_2017_119
crossref_primary_10_1016_j_celrep_2023_113603
crossref_primary_10_1007_s12195_020_00640_1
crossref_primary_10_1002_ijc_31332
crossref_primary_10_1016_j_isci_2020_101447
crossref_primary_10_1186_1471_2121_10_78
crossref_primary_10_1110_ps_062444807
crossref_primary_10_1007_s12257_020_0102_x
crossref_primary_10_1016_j_ejmech_2013_04_038
crossref_primary_10_1080_21691401_2018_1481863
crossref_primary_10_1021_ja211941d
crossref_primary_10_1007_s10549_014_2925_7
crossref_primary_10_2217_crc_12_62
crossref_primary_10_1080_14756366_2017_1370583
crossref_primary_10_3389_fimmu_2022_1006718
crossref_primary_10_3390_cells10051252
crossref_primary_10_1016_j_ccr_2008_02_019
crossref_primary_10_1016_j_ejmech_2017_09_061
crossref_primary_10_1080_07391102_2012_687525
crossref_primary_10_1038_srep03977
crossref_primary_10_1517_17425247_2016_1144588
crossref_primary_10_1016_j_yexcr_2008_10_024
crossref_primary_10_1128_MCB_00742_10
crossref_primary_10_1038_srep18430
crossref_primary_10_1039_C6CC06719K
crossref_primary_10_1091_mbc_E15_05_0269
crossref_primary_10_1021_cb300216f
crossref_primary_10_3390_ijms22020587
crossref_primary_10_1038_onc_2014_106
crossref_primary_10_1038_s41598_019_42969_2
crossref_primary_10_1016_j_str_2024_05_018
crossref_primary_10_1152_ajpgi_00365_2009
crossref_primary_10_1016_j_biochi_2020_07_010
crossref_primary_10_3390_ijms21249535
crossref_primary_10_3390_pharmaceutics14020241
crossref_primary_10_1038_jid_2014_164
crossref_primary_10_3390_ijms22073663
crossref_primary_10_1007_s11010_018_3417_6
crossref_primary_10_1002_pro_3871
crossref_primary_10_1371_journal_pone_0023240
crossref_primary_10_1016_j_cell_2017_09_017
crossref_primary_10_1074_jbc_RA118_004139
crossref_primary_10_1039_C4MB00471J
crossref_primary_10_1039_D1NR03180E
crossref_primary_10_1080_07357900701522653
crossref_primary_10_3389_fphar_2015_00283
crossref_primary_10_3892_or_2014_3102
crossref_primary_10_1146_annurev_biophys_37_032807_125829
crossref_primary_10_1073_pnas_1522367113
crossref_primary_10_1002_bies_20582
crossref_primary_10_1016_j_ceb_2020_01_016
crossref_primary_10_1111_bph_16185
crossref_primary_10_1021_acs_biochem_8b00471
crossref_primary_10_1016_j_nbd_2020_104961
crossref_primary_10_1182_blood_2011_02_337360
crossref_primary_10_3390_cryst10090725
crossref_primary_10_3390_s16060914
crossref_primary_10_1158_0008_5472_CAN_10_2384
crossref_primary_10_1073_pnas_1516373112
crossref_primary_10_1080_1061186X_2020_1853756
crossref_primary_10_1016_j_bbamem_2016_12_015
crossref_primary_10_1371_journal_pone_0193158
crossref_primary_10_1111_cbdd_12453
crossref_primary_10_1517_14728222_2011_648617
crossref_primary_10_1016_j_cell_2016_10_038
crossref_primary_10_1371_journal_pone_0264717
crossref_primary_10_1073_pnas_1518361112
crossref_primary_10_1038_cgt_2008_15
crossref_primary_10_7554_eLife_73218
Cites_doi 10.1016/j.febslet.2004.06.014
10.1016/1040-8428(94)00144-I
10.1016/S0092-8674(02)00940-6
10.1021/bi010037b
10.1159/000066203
10.1126/science.1082024
10.1038/nature01392
10.1016/S1535-6108(04)00083-2
10.1016/S0092-8674(02)00963-7
10.1126/science.1074611
10.1038/313144a0
10.1016/S1097-2765(03)00048-0
10.1016/S1097-2765(03)00047-9
10.1093/emboj/16.2.281
10.1074/jbc.M401244200
10.1074/jbc.271.11.6071
10.1074/jbc.M202880200
10.1016/S1097-2765(03)00350-2
10.1016/S0014-4827(02)00095-2
10.1016/S0014-4827(02)00104-0
10.1126/science.3798106
10.1016/S1535-6108(04)00122-9
10.1073/pnas.0307286101
10.1038/35077225
10.1016/S0092-8674(00)00114-8
10.1093/oxfordjournals.jbchem.a021718
10.1016/0092-8674(90)90801-K
10.1038/35052073
10.1093/emboj/19.17.4632
ContentType Journal Article
Copyright Copyright © 2005, American Society for Microbiology 2005
Copyright_xml – notice: Copyright © 2005, American Society for Microbiology 2005
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
5PM
DOI 10.1128/MCB.25.17.7734-7742.2005
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE



MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
Biology
EISSN 1098-5549
EndPage 7742
ExternalDocumentID 10_1128_MCB_25_17_7734_7742_2005
16107719
12269195
mcb_25_17_7734
Genre Signal Transduction
Research Support, U.S. Gov't, Non-P.H.S
Research Support, U.S. Gov't, P.H.S
Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NCI NIH HHS
  grantid: R01 CA079992
– fundername: NIAMS NIH HHS
  grantid: R01-AR051448
– fundername: NCI NIH HHS
  grantid: K01 CA092246
– fundername: NCI NIH HHS
  grantid: R01 CA079992-06
– fundername: NCI NIH HHS
  grantid: R01-CA079992
– fundername: NCI NIH HHS
  grantid: K01-CA092246
– fundername: NIAMS NIH HHS
  grantid: R01 AR051448
GroupedDBID ---
-DZ
-~X
.55
.GJ
0R~
123
18M
29M
2WC
39C
3O-
4.4
53G
5RE
5VS
9M8
AAPBV
AAUGY
ABPTK
ACGFO
ACKIV
ACNCT
ADBBV
ADIYS
AENEX
AFFNX
AGVNZ
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BTFSW
C1A
CS3
DIK
DU5
E3Z
EBS
EJD
F5P
GX1
H13
HH5
HYE
HZ~
IH2
KQ8
L7B
MVM
N9A
O9-
OK1
P2P
RHF
RHI
RNS
RPM
RSF
TFL
TFW
TR2
UCJ
UDS
VQA
W8F
WH7
WOQ
X7M
Y6R
ZA5
ZCA
ABJNI
ABRLO
ABTAH
AEOZL
AGHSJ
CGR
CUY
CVF
ECM
EIF
EMOBN
F20
M4Z
NPM
TDBHL
WHG
YYP
ZGI
ZXP
ZY4
AAYXX
CITATION
7X8
5PM
ID FETCH-LOGICAL-c626t-6be09205ca4ab58de2b91d5d9d0e99af375ce2c7a89bc0e304fa0af0bc93f6923
IEDL.DBID RPM
ISSN 0270-7306
1098-5549
IngestDate Tue Sep 17 21:21:59 EDT 2024
Sat Aug 17 03:30:52 EDT 2024
Thu Sep 12 19:36:23 EDT 2024
Sat Sep 28 07:49:14 EDT 2024
Tue Jun 13 19:24:49 EDT 2023
Wed May 18 15:26:14 EDT 2016
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 17
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c626t-6be09205ca4ab58de2b91d5d9d0e99af375ce2c7a89bc0e304fa0af0bc93f6923
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Corresponding author. Mailing address: Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 809C Stellar-Chance Laboratories, 422 Curie Boulevard, Philadelphia, PA 19104-6059. Phone: (215) 898-3072. Fax: (215) 573-4764. E-mail: mlemmon@mail.med.upenn.edu.
J.P.D. and M.B.B. contributed equally to this study.
OpenAccessLink https://doi.org/10.1128/mcb.25.17.7734-7742.2005
PMID 16107719
PQID 68495058
PQPubID 23479
PageCount 9
ParticipantIDs crossref_primary_10_1128_MCB_25_17_7734_7742_2005
informaworld_taylorfrancis_310_1128_MCB_25_17_7734_7742_2005
proquest_miscellaneous_68495058
pubmedcentral_primary_oai_pubmedcentral_nih_gov_1190273
highwire_asm_mcb_25_17_7734
pubmed_primary_16107719
PublicationCentury 2000
PublicationDate 20050901
9/1/2005
2005-Sep
2005-09-01
PublicationDateYYYYMMDD 2005-09-01
PublicationDate_xml – month: 09
  year: 2005
  text: 20050901
  day: 01
PublicationDecade 2000
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Molecular and Cellular Biology
PublicationTitleAlternate Mol Cell Biol
PublicationYear 2005
Publisher American Society for Microbiology
Taylor & Francis
Publisher_xml – name: American Society for Microbiology
– name: Taylor & Francis
References 2158859 - Cell. 1990 Apr 20;61(2):203-12
12620236 - Mol Cell. 2003 Feb;11(2):495-505
2981413 - Nature. 1985 Jan 10-18;313(5998):144-7
15093539 - Cancer Cell. 2004 Apr;5(4):317-28
10970856 - EMBO J. 2000 Sep 1;19(17):4632-43
8626392 - J Biol Chem. 1996 Mar 15;271(11):6071-6
12620237 - Mol Cell. 2003 Feb;11(2):507-17
12610629 - Nature. 2003 Feb 13;421(6924):756-60
11252954 - Nat Rev Mol Cell Biol. 2001 Feb;2(2):127-37
6318976 - Cancer Res. 1984 Feb;44(2):753-60
12648471 - Exp Cell Res. 2003 Mar 10;284(1):122-30
14527402 - Mol Cell. 2003 Sep;12(3):541-52
15016810 - J Biol Chem. 2004 May 21;279(21):22387-98
12297050 - Cell. 2002 Sep 20;110(6):775-87
14732693 - Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):923-8
12154198 - Science. 2002 Aug 23;297(5585):1330-3
11357143 - Nature. 2001 May 17;411(6835):355-65
9276679 - J Biochem. 1997 Jul;122(1):116-21
1503407 - Anticancer Res. 1992 Jul-Aug;12(4):1183-7
9029149 - EMBO J. 1997 Jan 15;16(2):281-94
11057895 - Cell. 2000 Oct 13;103(2):211-25
12730587 - Science. 2003 May 2;300(5620):750-2
12297049 - Cell. 2002 Sep 20;110(6):763-73
11467954 - Biochemistry. 2001 Jul 31;40(30):8930-9
12648472 - Exp Cell Res. 2003 Mar 10;284(1):131-9
12011054 - J Biol Chem. 2002 Aug 2;277(31):28330-9
3798106 - Science. 1987 Jan 9;235(4785):177-82
15144948 - Cancer Cell. 2004 May;5(5):411-5
7612182 - Crit Rev Oncol Hematol. 1995 Jul;19(3):183-232
15225657 - FEBS Lett. 2004 Jul 2;569(1-3):332-6
12422051 - Oncology. 2002;63 Suppl 1:17-24
Mattoon D. (R22) 2004; 101
Schlessinger J (R27) 2000; 103
Berezov A. (R3) 2002; 277
Ferguson K. M. (R12) 2003; 11
Burgess A. W. (R6) 2003; 12
Franklin M. C. (R14) 2004; 5
Blume-Jensen P. (R5) 2001; 411
Gorgoulis V. (R18) 1992; 12
Ferguson K. M. (R13) 2000; 19
Arteaga C. L (R1) 2003; 284
Schlessinger J (R28) 2003; 300
Yarden Y. (R32) 2001; 2
Libermann T. A. (R20) 1985; 313
Odaka M. (R23) 1997; 122
Elleman T. C. (R11) 2001; 40
Berger M. B. (R4) 2004; 569
Beerli R. R. (R2) 1996; 271
Ullrich A. (R30) 1990; 61
Cho H. S. (R8) 2002; 297
Ranson M. (R25) 2002; 63
Fry D. W (R15) 2003; 284
Slamon D. J. (R29) 1987; 235
Libermann T. A. (R21) 1984; 44
Dancey J. E (R10) 2004; 5
Ogiso H. (R24) 2002; 110
Walker F. (R31) 2004; 279
Cho H. S. (R9) 2003; 421
Salomon D. S. (R26) 1995; 19
Garrett T. P. J. (R16) 2003; 11
Garrett T. P. J. (R17) 2002; 110
Lemmon M. A. (R19) 1997; 16
Cantor C. R. (R7) 1980
References_xml – volume: 569
  start-page: 332
  year: 2004
  ident: R4
  publication-title: FEBS Lett.
  doi: 10.1016/j.febslet.2004.06.014
  contributor:
    fullname: Berger M. B.
– volume: 19
  start-page: 183
  year: 1995
  ident: R26
  publication-title: Crit. Rev. Oncol. Hematol.
  doi: 10.1016/1040-8428(94)00144-I
  contributor:
    fullname: Salomon D. S.
– volume: 110
  start-page: 763
  year: 2002
  ident: R17
  publication-title: Cell
  doi: 10.1016/S0092-8674(02)00940-6
  contributor:
    fullname: Garrett T. P. J.
– volume: 40
  start-page: 8930
  year: 2001
  ident: R11
  publication-title: Biochemistry
  doi: 10.1021/bi010037b
  contributor:
    fullname: Elleman T. C.
– volume: 63
  start-page: 17
  issue: 1
  year: 2002
  ident: R25
  publication-title: Oncology
  doi: 10.1159/000066203
  contributor:
    fullname: Ranson M.
– volume: 300
  start-page: 750
  year: 2003
  ident: R28
  publication-title: Science
  doi: 10.1126/science.1082024
  contributor:
    fullname: Schlessinger J
– volume: 421
  start-page: 756
  year: 2003
  ident: R9
  publication-title: Nature
  doi: 10.1038/nature01392
  contributor:
    fullname: Cho H. S.
– volume: 5
  start-page: 317
  year: 2004
  ident: R14
  publication-title: Cancer Cell
  doi: 10.1016/S1535-6108(04)00083-2
  contributor:
    fullname: Franklin M. C.
– volume: 110
  start-page: 775
  year: 2002
  ident: R24
  publication-title: Cell
  doi: 10.1016/S0092-8674(02)00963-7
  contributor:
    fullname: Ogiso H.
– volume: 297
  start-page: 1330
  year: 2002
  ident: R8
  publication-title: Science
  doi: 10.1126/science.1074611
  contributor:
    fullname: Cho H. S.
– volume: 313
  start-page: 144
  year: 1985
  ident: R20
  publication-title: Nature
  doi: 10.1038/313144a0
  contributor:
    fullname: Libermann T. A.
– volume: 11
  start-page: 495
  year: 2003
  ident: R16
  publication-title: Mol. Cell
  doi: 10.1016/S1097-2765(03)00048-0
  contributor:
    fullname: Garrett T. P. J.
– volume: 12
  start-page: 1183
  year: 1992
  ident: R18
  publication-title: Anticancer Res.
  contributor:
    fullname: Gorgoulis V.
– volume: 44
  start-page: 753
  year: 1984
  ident: R21
  publication-title: Cancer Res.
  contributor:
    fullname: Libermann T. A.
– volume: 11
  start-page: 507
  year: 2003
  ident: R12
  publication-title: Mol. Cell
  doi: 10.1016/S1097-2765(03)00047-9
  contributor:
    fullname: Ferguson K. M.
– volume: 16
  start-page: 281
  year: 1997
  ident: R19
  publication-title: EMBO J.
  doi: 10.1093/emboj/16.2.281
  contributor:
    fullname: Lemmon M. A.
– volume: 279
  start-page: 22387
  year: 2004
  ident: R31
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M401244200
  contributor:
    fullname: Walker F.
– volume: 271
  start-page: 6071
  year: 1996
  ident: R2
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.271.11.6071
  contributor:
    fullname: Beerli R. R.
– volume: 277
  start-page: 28330
  year: 2002
  ident: R3
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M202880200
  contributor:
    fullname: Berezov A.
– volume: 12
  start-page: 541
  year: 2003
  ident: R6
  publication-title: Mol. Cell
  doi: 10.1016/S1097-2765(03)00350-2
  contributor:
    fullname: Burgess A. W.
– volume: 284
  start-page: 131
  year: 2003
  ident: R15
  publication-title: Exp. Cell Res.
  doi: 10.1016/S0014-4827(02)00095-2
  contributor:
    fullname: Fry D. W
– volume: 284
  start-page: 122
  year: 2003
  ident: R1
  publication-title: Exp. Cell Res.
  doi: 10.1016/S0014-4827(02)00104-0
  contributor:
    fullname: Arteaga C. L
– volume: 235
  start-page: 177
  year: 1987
  ident: R29
  publication-title: Science
  doi: 10.1126/science.3798106
  contributor:
    fullname: Slamon D. J.
– volume-title: Biophysical chemistry: techniques for the study of biological structure and function. W. H
  year: 1980
  ident: R7
  contributor:
    fullname: Cantor C. R.
– volume: 5
  start-page: 411
  year: 2004
  ident: R10
  publication-title: Cancer Cell
  doi: 10.1016/S1535-6108(04)00122-9
  contributor:
    fullname: Dancey J. E
– volume: 101
  start-page: 923
  year: 2004
  ident: R22
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0307286101
  contributor:
    fullname: Mattoon D.
– volume: 411
  start-page: 355
  year: 2001
  ident: R5
  publication-title: Nature
  doi: 10.1038/35077225
  contributor:
    fullname: Blume-Jensen P.
– volume: 103
  start-page: 211
  year: 2000
  ident: R27
  publication-title: Cell
  doi: 10.1016/S0092-8674(00)00114-8
  contributor:
    fullname: Schlessinger J
– volume: 122
  start-page: 116
  year: 1997
  ident: R23
  publication-title: J. Biochem.
  doi: 10.1093/oxfordjournals.jbchem.a021718
  contributor:
    fullname: Odaka M.
– volume: 61
  start-page: 203
  year: 1990
  ident: R30
  publication-title: Cell
  doi: 10.1016/0092-8674(90)90801-K
  contributor:
    fullname: Ullrich A.
– volume: 2
  start-page: 127
  year: 2001
  ident: R32
  publication-title: Nat. Rev. Mol. Cell. Biol.
  doi: 10.1038/35052073
  contributor:
    fullname: Yarden Y.
– volume: 19
  start-page: 4632
  year: 2000
  ident: R13
  publication-title: EMBO J.
  doi: 10.1093/emboj/19.17.4632
  contributor:
    fullname: Ferguson K. M.
SSID ssj0006903
Score 2.3623054
Snippet Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
Structural studies have shown that ligand-induced epidermal growth factor receptor (EGFR) dimerization involves major domain rearrangements that expose a...
SourceID pubmedcentral
proquest
crossref
pubmed
informaworld
highwire
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 7734
SubjectTerms Binding Sites
Dimerization
Enzyme Activation
Ligands
Models, Molecular
Molecular Sequence Data
Mutation - genetics
Protein Structure, Quaternary
Protein Structure, Tertiary
Receptor, Epidermal Growth Factor - chemistry
Receptor, Epidermal Growth Factor - genetics
Receptor, Epidermal Growth Factor - metabolism
Signal Transduction
Surface Plasmon Resonance
Transforming Growth Factor alpha - pharmacology
Title Epidermal Growth Factor Receptor Dimerization and Activation Require Ligand-Induced Conformational Changes in the Dimer Interface
URI http://mcb.asm.org/content/25/17/7734.abstract
https://www.tandfonline.com/doi/abs/10.1128/MCB.25.17.7734-7742.2005
https://www.ncbi.nlm.nih.gov/pubmed/16107719
https://search.proquest.com/docview/68495058
https://pubmed.ncbi.nlm.nih.gov/PMC1190273
Volume 25
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwEB21lRBcUClfaQv4wDXZJI7jWOLSLl0qRBEHKvVmOf6gkbrpik0PHPvPGTsx3VZISNwixXESvYnnTTwzD-C9obUSFBGokdynlTM6FcaYVKOrVbhqWm5CtsXX-vS8-nzBLraAxVqYkLSv2y7rr5ZZ312G3MrVUs9intjs29m8QC-Gbne2Dduc0hiiT8svhnthW7nkeYrmW8f0nbKZnc2Ps5JlBc84pxUSyyq0ogzqNUgjOPf9djbdU2wZ_KCP6d_Y6MOkyg0vtdiFpxO9JEfjazyDLdvvwaNRcPLXHjyeR32353B74sVh8WZX5BPG4sMlWQTtHYJM0q78wcfOb-eMdZpE9YYc6SiGhoN8CrElX7ofeCb1CiDaGuILCGM5JE481i6sSdcTJJrjhCT8hHRK2xdwvjj5Pj9NJ0EGD109pHVrc1HmTKtKtawxtmxFYZgRJrdCKEc507bUXDWi1bmleeVUrlzeakEdGgN9CTv9dW9fA3FIXZhwxhfW4lqNNKl1jXHU1k1hFSsTKCIOcjX23ZAhXikbiTDKksmCSw-j9DB6NU2WwEEETKr1Ui51uzEugQ-bGMoh_BFxo3yJpP-e_F3EXCJQfltF9fb6Zi3rBoPMnDUJvBot4O6BJ6NKgN-zjT8DfG_v-2fQ5EOP78nE9__7ygN4ErrMhnS4Q9gZft7YN8ifhvZt-F5-A6mNGD0
link.rule.ids 230,315,733,786,790,891,27957,27958,53827,53829
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEB6VIlQuPMrLFOgeuNrxa722xKWEhgBJxaFFva3W-6AWjRsR5wA3_jmzay-kERKCW6TdrOLsZ8833m_mA3ipskJUGe5AgeQ-zI2SYaWUCiWGWoFPTc2UU1ucFNOz_P05Pd8B6mthnGhf1k3UXi6itrlw2srlQo68Tmz0cT5OMIph2B3dgJt4v6bMJ-nDAxgTPnewnLI4RAAXXsCTlqP5-HWU0ihhEWNZjtQyd80onX8NEgnGbMedzQDlmwZvdTL9Ex_dllVuxKnJXfjkr7CXp3yJ1l0dye9bzR__-S-4B3cG5kqO-uH7sKPbfbjVe1l-24e9sbeOewA_jq3vLF7FJXmLaX53QSbO1ocgSdVL--FNY0-K-hJQIlpFjqT3WcNJVp2syaz5jCOhNReRWhFbm-grLXHhvixiRZqWIIftFyTu_aYRUj-Es8nx6XgaDl4PFhVFFxa1jqs0plLkoqal0mldJYqqSsW6qoTJGJU6lUyUVS1jncW5EbEwcS2rzCDOskew2161-gkQg6yIVkbZml0MA8jAalMqk-miTLSgaQCJ32C-7Ft6cJcKpSVHfPCU8oRxiw9u8WGNOmkABx4JXKwWfCHrjXkBvNoEB-_cyxbTO6Pw7O-LH3owcdwoe2IjWn21XvGixPw1pmUAj3to_f7BA1oDYNdA92uCbRt-fQSh5NqHD9B5-t_fPIS96el8xmfvTj4cwG3XzNap7p7Bbvd1rZ8jTevqF-6m_AnLhTos
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LbtQwFLWgiMeGR4ESCtQLtsnk5TiW2JRphwJt1QWVKjaWnzRqJx0xmQXs-HOunRjSERJSd5HisZLxSe658b3nIPRWF5VgBaxABeQ-Lq1WMdNaxwpCrYC3pqHaV1scVwen5aczcjay-vJF-0o2SXs5T9rm3NdWLuZqEurEJidH0wyiGITdyULbyW10B57ZnIVEfXgJQ9LnN5dzmsYA4ioU8eT15Gj6PslJktGE0qIEell6QUrvYQNkglKnujMOUkE4eE3N9F-cdL20chSrZo_Q13CXfYnKRbLqZKJ-rglA3uhveIweDgwW7_ZDnqBbpt1Ed3tPyx-b6P40WMg9Rb_2nf8s3Mkl_gDpfneOZ97eBwNZNQt3sNe4HaO-FRSLVuNdFfzWYJCrUjb4sPkGZ2JnMqKMxq5HMXRcwsR9e8QSNy0GLttPiP13TiuUeYZOZ_tfpgfx4Png0FF1cSVNyvKUKFEKSWptcskyTTTTqWFM2IISZXJFRc2kSk2RllakwqZSscIC3ornaKO9as0LhC2wI8Ksdr27EA6AiUlba1uYqs6MIHmEsrDIfNFLe3CfEuU1B4zwnPCMcocR7jDiDDtJhLYDGrhYzvlcydG4CL0bA4R3_qOL7R1SePH_yXcCoDgslNu5Ea25Wi15VUMem5I6Qls9vP5e8IDYCNFrwPszwMmHXz8DcPIy4gN8Xt74lzvo3snejB9-PP68jR54TVtffPcKbXTfV-Y1sLVOvvHP5W-b4Dys
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Epidermal+growth+factor+receptor+dimerization+and+activation+require+ligand-induced+conformational+changes+in+the+dimer+interface&rft.jtitle=Molecular+and+cellular+biology&rft.au=Dawson%2C+Jessica+P&rft.au=Berger%2C+Mitchell+B&rft.au=Lin%2C+Chun-Chi&rft.au=Schlessinger%2C+Joseph&rft.date=2005-09-01&rft.issn=0270-7306&rft.volume=25&rft.issue=17&rft.spage=7734&rft_id=info:doi/10.1128%2FMCB.25.17.7734-7742.2005&rft_id=info%3Apmid%2F16107719&rft.externalDocID=16107719
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0270-7306&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0270-7306&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0270-7306&client=summon