Conformational biosensors reveal GPCR signalling from endosomes

• Published in: Nature (London) Vol. 495; no. 7442; pp. 534 - 538
• Main Authors: Irannejad, Roshanak, Tomshine, Jin C., Tomshine, Jon R., Chevalier, Michael, Mahoney, Jacob P., Steyaert, Jan, Rasmussen, Søren G. F., Sunahara, Roger K., El-Samad, Hana, Huang, Bo, von Zastrow, Mark
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.03.2013; Nature Publishing Group
• Subjects: 631/80/86/2363; Adrenergic beta-2 Receptor Agonists - pharmacology; Biosensing Techniques - methods; Biosensors; Cell Membrane - chemistry; Cell Membrane - metabolism; Clathrin-Coated Vesicles; Cyclic AMP - metabolism; Cytoplasm; Dopamine; Endocytosis; Endosomes - chemistry; Endosomes - metabolism; Green Fluorescent Proteins - analysis; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; GTP-Binding Protein alpha Subunits, Gs - metabolism; HEK293 Cells; Humanities and Social Sciences; Humans; Hypotheses; Isoproterenol - pharmacology; letter; Ligands (Biochemistry); Models, Biological; multidisciplinary; Pharmacology; Phosphorylation; Plasma; Protein Conformation; Proteins; Receptors, Adrenergic, beta-2 - chemistry; Receptors, Adrenergic, beta-2 - immunology; Receptors, Adrenergic, beta-2 - metabolism; Recruitment; Science; Signal Transduction; Single-Domain Antibodies - genetics; Single-Domain Antibodies - immunology; United States

Conformation-specific antibodies capable of monitoring the activation state of a G-protein-coupled seven-transmembrane receptor, the β 2 -adrenoceptor, reveals receptor and G-protein activation not only in the plasma membrane, but also in the endosome. Adrenoceptor signalling linked to endosomes It...