Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts

We established a new method of transient expression using tomato fruit protoplasts. The method showed that L-ascorbic acid (AsA) content in tomato protoplasts was increased by transient expression of the L-galactose-1-phosphate phosphatase gene. This system provides a means of rapid analysis to clar...

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Published inBioscience, biotechnology, and biochemistry Vol. 77; no. 3; pp. 673 - 675
Main Authors SAKAMOTO, Shingo, FUJIKAWA, Yukichi, ESAKA, Muneharu
Format Journal Article
LanguageEnglish
Published England Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013
Oxford University Press
Subjects
ascorbic acid
Ascorbic Acid - biosynthesis
Fruit - cytology
Gene Expression
Genetic Engineering - methods
Lycopersicon esculentum
Nicotiana - enzymology
Nicotiana - genetics
Phosphoric Monoester Hydrolases - genetics
protoplast
Protoplasts - metabolism
Solanum lycopersicum - cytology
Solanum lycopersicum - genetics
Time Factors
tomato fruit
transient gene expression
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Abstract We established a new method of transient expression using tomato fruit protoplasts. The method showed that L-ascorbic acid (AsA) content in tomato protoplasts was increased by transient expression of the L-galactose-1-phosphate phosphatase gene. This system provides a means of rapid analysis to clarify the function of AsA biosynthetic enzymes and AsA roles in tomato fruit.
AbstractList We established a new method of transient expression using tomato fruit protoplasts. The method showed that L-ascorbic acid (AsA) content in tomato protoplasts was increased by transient expression of the L-galactose-1-phosphate phosphatase gene. This system provides a means of rapid analysis to clarify the function of AsA biosynthetic enzymes and AsA roles in tomato fruit.
Author FUJIKAWA, Yukichi
SAKAMOTO, Shingo
ESAKA, Muneharu
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  fullname: ESAKA, Muneharu
  organization: Graduate School of Biosphere Sciences, Hiroshima University
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Snippet We established a new method of transient expression using tomato fruit protoplasts. The method showed that L-ascorbic acid (AsA) content in tomato protoplasts...
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SubjectTerms ascorbic acid
Ascorbic Acid - biosynthesis
Fruit - cytology
Gene Expression
Genetic Engineering - methods
Lycopersicon esculentum
Nicotiana - enzymology
Nicotiana - genetics
Phosphoric Monoester Hydrolases - genetics
protoplast
Protoplasts - metabolism
Solanum lycopersicum - cytology
Solanum lycopersicum - genetics
Time Factors
tomato fruit
transient gene expression
Title Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts
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