Degenerate sequence-based CRISPR diagnostic for Crimean-Congo hemorrhagic fever virus

• Published in: PLoS neglected tropical diseases Vol. 16; no. 3; p. e0010285
• Main Authors: Li, Hongzhao, Bello, Alexander, Smith, Greg, Kielich, Dominic M S, Strong, James E, Pickering, Bradley S
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.03.2022; Public Library of Science (PLoS)
• Subjects: Acids; Biology and Life Sciences; Biosafety; Care and treatment; Climate change; COVID-19; Crimean hemorrhagic fever; CRISPR; Cross-reactivity; Defence mechanisms; Dengue fever; Deoxyribonucleic acid; Detection; Diagnosis; DNA; Engineering and Technology; Epidemics; Fever; Gene editing; Genomes; Geographical distribution; Haemorrhage; Hemorrhage; Hemorrhagic Fever Virus, Crimean-Congo - genetics; Hemorrhagic Fever, Crimean; Humans; Infectious diseases; Medical laboratories; Medicine and health sciences; Microbiological strains; Mutation; Mutations; Nucleic acids; Nucleotide sequence; Nucleotides; Pathogens; Plasmids; Polymerase chain reaction; Prokaryotes; Research and Analysis Methods; Ribonucleic acid; Risk factors; RNA; RNA polymerase; RNA viruses; RNA, Viral - genetics; Sequencing; Severe acute respiratory syndrome coronavirus 2; Spacer; Strains; Strains (organisms); Target detection; Testing; Thermal cycling; Tropical diseases; Viruses; United States; Europe

CRISPR (clustered regularly interspaced short palindromic repeats), an ancient defense mechanism used by prokaryotes to cleave nucleic acids from invading viruses and plasmids, is currently being harnessed by researchers worldwide to develop new point-of-need diagnostics. In CRISPR diagnostics, a CR...