Naive Human Pluripotent Cells Feature a Methylation Landscape Devoid of Blastocyst or Germline Memory

• Published in: Cell stem cell Vol. 18; no. 3; pp. 323 - 329
• Main Authors: Pastor, William A., Chen, Di, Liu, Wanlu, Kim, Rachel, Sahakyan, Anna, Lukianchikov, Anastasia, Plath, Kathrin, Jacobsen, Steven E., Clark, Amander T.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.03.2016
• Subjects: Animals; Blastocyst - cytology; Blastocyst - metabolism; Cell Line; DNA Methylation; Human Embryonic Stem Cells - cytology; Human Embryonic Stem Cells - metabolism; Humans; Mice; Oocytes - cytology; Oocytes - metabolism; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - metabolism; Stage-Specific Embryonic Antigens - metabolism

Human embryonic stem cells (hESCs) typically exhibit “primed” pluripotency, analogous to stem cells derived from the mouse post-implantation epiblast. This has led to a search for growth conditions that support self-renewal of hESCs akin to hypomethylated naive epiblast cells in human pre-implantati...