Laboratory challenges of Plasmodium species identification in Aceh Province, Indonesia, a malaria elimination setting with newly discovered P. knowlesi

• Published in: PLoS neglected tropical diseases Vol. 12; no. 11; p. e0006924
• Main Authors: Coutrier, Farah N., Tirta, Yusrifar K., Cotter, Chris, Zarlinda, Iska, González, Iveth J., Schwartz, Alanna, Maneh, Cut, Marfurt, Jutta, Murphy, Maxwell, Herdiana, Herdiana, Anstey, Nicholas M., Greenhouse, Bryan, Hsiang, Michelle S., Noviyanti, Rintis
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.11.2018; Public Library of Science (PLoS)
• Subjects: Biology and Life Sciences; Control; Cross-reactivity; Cytochrome; Cytochrome b; Cytochromes; Detection; Diagnosis; Disease Eradication; DNA; Endemism; Epidemiology; Gene sequencing; Genes; Genetic aspects; Health; Health care facilities; Health surveillance; Human diseases; Humans; Identification; Identification methods; Indonesia - epidemiology; Infection; Infections; Laboratories; Malaria; Malaria - epidemiology; Malaria - parasitology; Malaria - prevention & control; Medical research; Medical tests; Medicine and Health Sciences; Methods; Microscopy; Molecular biology; Mortality; Nucleic Acid Amplification Techniques; Nucleic acids; Nucleotide sequence; Parasites; Pathogenesis; PCR; Pediatrics; People and Places; Plasmodium; Plasmodium (Protozoa); Plasmodium - classification; Plasmodium - genetics; Plasmodium - isolation & purification; Plasmodium falciparum; Plasmodium knowlesi - classification; Plasmodium knowlesi - genetics; Plasmodium knowlesi - isolation & purification; Polymerase Chain Reaction; Prevention; Public health; Quality assurance; Quality control; R&D; Research & development; Research and Analysis Methods; Ribosomal RNA; RNA; rRNA 18S; Samples; Species; Species identification; Surveillance; Testing; Tropical diseases; Vector-borne diseases; Zoonoses; Indonesia; Borneo; Myanmar (Burma); United States > US; Malaysia; California; Thailand; Jakarta Indonesia; San Francisco California; Banda Aceh Indonesia; Asia; Sumatra; Australia
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0006924

The discovery of the life-threatening zoonotic infection Plasmodium knowlesi has added to the challenges of prompt and accurate malaria diagnosis and surveillance. In this study from Aceh Province, Indonesia, a malaria elimination setting where P. knowlesi endemicity was not previously known, we rep...