Argonaute-1 binds transcriptional enhancers and controls constitutive and alternative splicing in human cells

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 111; no. 44; pp. 15622 - 15629
• Main Authors: Alló, Mariano, Agirre, Eneritz, Bessonov, Sergey, Bertucci, Paola, Acuña, Luciana Gómez, Buggiano, Valeria, Bellora, Nicolás, Singh, Babita, Petrillo, Ezequiel, Blaustein, Matías, Miñana, Belén, Dujardin, Gwendal, Pozzi, Berta, Pelisch, Federico, Bechara, Elías, Agafonov, Dmitry E., Srebrow, Anabella, Lührmann, Reinhard, Valcárcel, Juan, Eyras, Eduardo, Kornblihtt, Alberto R.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 04.11.2014; National Acad Sciences
• Series: Inaugural Article
• Subjects: Alternative splicing; Alternative Splicing - physiology; Antibodies; Argonaute Proteins - genetics; Argonaute Proteins - metabolism; Biological Sciences; Cell Line; Cells; Chromatin; cytoplasm; Enhancer Elements, Genetic - physiology; eukaryotic cells; Eukaryotic Initiation Factors - genetics; Eukaryotic Initiation Factors - metabolism; Exons; Gene Expression Regulation - physiology; Genes; Genomics; Histones; Humans; Introns; messenger RNA; Neurons; Proteins; RNA; RNA - genetics; RNA - metabolism; RNA interference; RNA polymerase; Sequence Analysis, RNA; Splicing; transcription (genetics); Transcription, Genetic - physiology; translation (genetics); alternative splicing; Argonaute proteins; transcriptional enhancers

Significance Argonaute proteins are well characterized factors in posttranscriptional gene silencing, the process by which small RNAs trigger mRNA degradation or inhibit translation in the cytoplasm. We report here that Argonaute proteins also play important roles in the nucleus. Our genome-wide ana...