High-throughput, detailed, cell-specific neuroanatomy of dendritic spines using microinjection and confocal microscopy

• Published in: Nature protocols Vol. 6; no. 9; pp. 1391 - 1411
• Main Authors: Dumitriu, Dani, Rodriguez, Alfredo, Morrison, John H
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.09.2011; Nature Publishing Group
• Subjects: 631/1647/1407; 631/1647/245/2225; 631/1647/328/1978; 631/378/2597; Aging; Analytical Chemistry; Animals; Biological Techniques; Biomedical and Life Sciences; Collaboration; Computational Biology/Bioinformatics; Dendritic Spines - ultrastructure; Dyes; Fluorescent Dyes - analysis; Haplorhini; Laboratories; Life Sciences; Male; Medicine; Mice; Microarrays; Microinjections - methods; Microscopy; Microscopy, Confocal - methods; Microscopy, Electron, Transmission; Neurosciences; Organic Chemistry; Plasticity; protocol; Rats; Software; Tissue Preservation - methods; New York; United States > US

Morphological features such as size, shape and density of dendritic spines have been shown to reflect important synaptic functional attributes and potential for plasticity. Here we describe in detail a protocol for obtaining detailed morphometric analysis of spines using microinjection of fluorescen...