survey of human brain transcriptome diversity at the single cell level

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 23; pp. 7285 - 7290
• Main Authors: Darmanis, Spyros, Sloan, Steven A., Zhang, Ye, Enge, Martin, Caneda, Christine, Shuer, Lawrence M., Gephart, Melanie G. Hayden, Barres, Ben A., Quake, Stephen R.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 09.06.2015; National Acad Sciences
• Subjects: Adult; adults; Biological Sciences; Brain; Brain - cytology; Brain - embryology; Brain - metabolism; Cells; HLA Antigens - immunology; Human subjects; Humans; major histocompatibility complex; Neurons; Neurons - cytology; Neurons - immunology; Ribonucleic acid; RNA; Sequence Analysis, RNA; Single-Cell Analysis; surveys; Transcriptome; transcriptomics; human brain; single cells; RNAseq; neurons; interneurons

Significance The brain comprises an immense number of cells and cellular connections. We describe the first, to our knowledge, single cell whole transcriptome analysis of human adult cortical samples. We have established an experimental and analytical framework with which the complexity of the human...