Engineering of a single-chain variable-fragment (scFv) antibody specific for the stolbur phytoplasma (mollicute) and its expression in Escherichia coli and tobacco plants

• Published in: Applied and Environmental Microbiology Vol. 64; no. 11; pp. 4566 - 4572
• Main Authors: Le Gall, F. (Institut National de la Recherche Agronomique, France.), Bove, J.M, Garnier, M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.11.1998
• Subjects: Agrobacterium tumefaciens; Agronomy. Soil science and plant productions; Bacteria; Biological and medical sciences; Biotechnology; DISEASE RESISTANCE; Ecology, environment; ESCHERICHIA COLI; FITOPLASMAS; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genetic engineering; GENETIC RESISTANCE; Genetic technics; Genetics and breeding of economic plants; IMMUNOGLOBULIN STRUCTURAL GENES; IMMUNOGLOBULINE; IMMUNOGLOBULINS; INMUNOGLOBULINA; Life Sciences; Methods. Procedures. Technologies; Microbiology; NICOTIANA TABACUM; Pest resistance; PHYTOPLASMAS; PHYTOPLASME; Plant Microbiology; Plant pathogens; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; RESISTANCE AUX MALADIES; RESISTANCE GENETIQUE; RESISTENCIA A LA ENFERMEDAD; RESISTENCIA GENETICA; Tobacco; Transgenic animals and transgenic plants; TRANSGENIC PLANTS; Varietal selection. Specialized plant breeding, plant breeding aims; Genetic transformation; Plant pathogen; Escherichia coli; Fv-Fragment; IgG1; Gene expression; Nicotiana tabacum; Antigen; Mollicutes; Resistance; Binding capacity; Gene; Dicotyledones; Angiospermae; Bacteria; Spermatophyta; Transgenic plant; Solanaceae; Recombinant protein; Molecular cloning; Enterobacteriaceae; Single chain antibody

From a hybridoma cell line (2A10) producing an immunoglobulin G1 directed against the major membrane protein of the stolbur phytoplasma, we have engineered scFv (single-chain variable-fragment) antibodies from the variable heavy (VH) and light (VL) domains of the immunoglobulin. The scFv gene was cl...