Tubular cell and keratinocyte single-cell transcriptomics applied to lupus nephritis reveal type I IFN and fibrosis relevant pathways

• Published in: Nature immunology Vol. 20; no. 7; pp. 915 - 927
• Main Authors: Der, Evan, Suryawanshi, Hemant, Morozov, Pavel, Kustagi, Manjunath, Goilav, Beatrice, Ranabothu, Saritha, Izmirly, Peter, Clancy, Robert, Belmont, H. Michael, Koenigsberg, Mordecai, Mokrzycki, Michele, Rominieki, Helen, Graham, Jay A., Rocca, Juan P., Bornkamp, Nicole, Jordan, Nicole, Schulte, Emma, Wu, Ming, Pullman, James, Slowikowski, Kamil, Raychaudhuri, Soumya, Guthridge, Joel, James, Judith, Buyon, Jill, Tuschl, Thomas, Putterman, Chaim
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.07.2019; Nature Publishing Group
• Subjects: 631/250/2520; 631/250/38; 692/420; Autoimmune diseases; Biomedical and Life Sciences; Biomedicine; Biopsy; Care and treatment; Cell Lineage - genetics; Cell proliferation; Computational Biology - methods; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - metabolism; Fibrosis; Gene Expression Profiling - methods; Heterogeneity; Humans; Immunology; Infectious Diseases; Interferon; Interferon Type I - metabolism; Keratinocytes; Keratinocytes - metabolism; Kidney Tubules - cytology; Kidney Tubules - metabolism; Lupus; Lupus nephritis; Lupus Nephritis - genetics; Lupus Nephritis - metabolism; Lupus Nephritis - pathology; Morbidity; Nephritis; Protein Binding; Resource; Ribonucleic acid; RNA; Signal Transduction; Single-Cell Analysis; Skin; Skin - immunology; Skin - metabolism; Skin - pathology; Systemic lupus erythematosus; Transcriptome; California

The molecular and cellular processes that lead to renal damage and to the heterogeneity of lupus nephritis (LN) are not well understood. We applied single-cell RNA sequencing (scRNA-seq) to renal biopsies from patients with LN and evaluated skin biopsies as a potential source of diagnostic and progn...