Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects

• Published in: Cell reports (Cambridge) Vol. 36; no. 2; p. 109353
• Main Authors: Jennewein, Madeleine F., MacCamy, Anna J., Akins, Nicholas R., Feng, Junli, Homad, Leah J., Hurlburt, Nicholas K., Seydoux, Emilie, Wan, Yu-Hsin, Stuart, Andrew B., Edara, Venkata Viswanadh, Floyd, Katharine, Vanderheiden, Abigail, Mascola, John R., Doria-Rose, Nicole, Wang, Lingshu, Yang, Eun Sung, Chu, Helen Y., Torres, Jonathan L., Ozorowski, Gabriel, Ward, Andrew B., Whaley, Rachael E., Cohen, Kristen W., Pancera, Marie, McElrath, M. Juliana, Englund, Janet A., Finzi, Andrés, Suthar, Mehul S., McGuire, Andrew T., Stamatatos, Leonidas
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 13.07.2021; Elsevier; Cell Press
• Subjects: 60 APPLIED LIFE SCIENCES; Angiotensin-Converting Enzyme 2 - chemistry; Angiotensin-Converting Enzyme 2 - immunology; Animals; Antibodies, Monoclonal - immunology; Antibodies, Neutralizing - immunology; Antibodies, Viral - chemistry; Antibodies, Viral - immunology; B.1.351; Binding Sites; Cell Line; COVID-19 - immunology; COVID-19 - prevention & control; Cross Reactions; CV3-25; Epitopes - immunology; Female; HEK293 Cells; Humans; Mice; monoclonal antibodies; neutralization; Neutralization Tests; NTD; Protein Binding - immunology; Protein Domains; RBD; S2 subunit; SARS-CoV-1; SARS-CoV-2; SARS-CoV-2 - immunology; Spike Glycoprotein, Coronavirus - chemistry; Spike Glycoprotein, Coronavirus - immunology; S2 subunit; SARS-CoV-2; SARS-CoV-1; B.1.351; RBD; neutralization; CV3-25; NTD; monoclonal antibodies

SARS-CoV-2 is one of three coronaviruses that have crossed the animal-to-human barrier and caused widespread disease in the past two decades. The development of a universal human coronavirus vaccine could prevent future pandemics. We characterize 198 antibodies isolated from four COVID-19+ subjects...