Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2

• Published in: PloS one Vol. 16; no. 3; p. e0248348
• Main Authors: Neerukonda, Sabari Nath, Vassell, Russell, Herrup, Rachel, Liu, Shufeng, Wang, Tony, Takeda, Kazuyo, Yang, Ye, Lin, Tsai-Lien, Wang, Wei, Weiss, Carol D.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.03.2021; Public Library of Science (PLoS)
• Subjects: ACE inhibitors; ACE2; Acquired immune deficiency syndrome; AIDS; Angiotensin-converting enzyme 2; Angiotensin-Converting Enzyme 2 - genetics; Angiotensin-Converting Enzyme 2 - immunology; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Biology and life sciences; Coronaviruses; COVID-19; COVID-19 - metabolism; Diagnosis; Drug Evaluation, Preclinical - methods; Editing; Enzymatic activity; Epidemics; Epidemiology; Evaluation; Fireflies; Fluorescence; Gag protein; Genetic aspects; Glycoproteins; HEK293 Cells; HIV; Human immunodeficiency virus; Humans; Lentivirus - metabolism; Leukemia; Medicine and health sciences; Methodology; Neon; Neutralization; Neutralization Tests - methods; Plasmids; Prevention; Proteases; Regulatory agencies; Research and analysis methods; Research facilities; SARS-CoV-2 - metabolism; SARS-CoV-2 - pathogenicity; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - genetics; Stomatitis; Testing; United States; Vaccines; Viruses; United States; United States > US; Maryland

Pseudoviruses are useful surrogates for highly pathogenic viruses because of their safety, genetic stability, and scalability for screening assays. Many different pseudovirus platforms exist, each with different advantages and limitations. Here we report our efforts to optimize and characterize an H...