Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2 - Neerukonda, Sabari Nath; Vassell, Russell; Herrup, Rachel; Liu, Shufeng; Wang, Tony; Takeda, Kazuyo; Yang, Ye; Lin, Tsai-Lien; Wang, Wei; Weiss, Carol D. (PloS one, 2021)
Keywords: ACE inhibitors; ACE2; Acquired immune deficiency syndrome; AIDS; Angiotensin-converting enzyme 2; Angiotensin-Converting Enzyme 2 - genetics; Angiotensin-Converting Enzyme 2 - immunology; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Biology and life sciences; Coronaviruses; COVID-19; COVID-19 - metabolism; Diagnosis; Drug Evaluation, Preclinical - methods; Editing; Enzymatic activity; Epidemics; Epidemiology; Evaluation; Fireflies; Fluorescence; Gag protein; Genetic aspects; Glycoproteins; HEK293 Cells; HIV; Human immunodeficiency virus; Humans; Lentivirus - metabolism; Leukemia; Medicine and health sciences; Methodology; Neon; Neutralization; Neutralization Tests - methods; Plasmids; Prevention; Proteases; Regulatory agencies; Research and analysis methods; Research facilities; SARS-CoV-2 - metabolism; SARS-CoV-2 - pathogenicity; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - genetics; Stomatitis; Testing; United States; Vaccines; Viruses; United States; United States--US; Maryland