Kinetic analysis of the transphosphorylation with creatine kinase by pressure-assisted capillary electrophoresis/dynamic frontal analysis

• Published in: Analytical and bioanalytical chemistry Vol. 413; no. 5; pp. 1453 - 1460
• Main Authors: Mine, Masanori, Mizuguchi, Hitoshi, Takayanagi, Toshio
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2021; Springer; Springer Nature B.V
• Subjects: Adenosine diphosphate; Analysis; Analytical Chemistry; Animals; Biochemistry; Capillaries; Capillary electrophoresis; Capillary pressure; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Creatine; Creatine - metabolism; Creatine kinase; Creatine Kinase, MM Form - metabolism; Dynamics; Electrophoresis; Electrophoresis, Capillary - instrumentation; Equipment Design; Food Science; Kinases; Kinetics; Laboratory Medicine; Methods; Monitoring/Environmental Analysis; phosphates; Phosphocreatine; Phosphocreatine - metabolism; Phosphorylation; Rabbits; Research Paper; Substrates; Japan; Transphosphorylation; Kinetic analysis; Dynamic frontal analysis; Capillary electrophoresis; Creatine kinase

Kinetic reactions of the transphosphorylation with creatine kinase (CK) were individually investigated between creatine (Cr) and creatine phosphate (CrP) by pressure-assisted capillary electrophoresis/dynamic frontal analysis (pCE/DFA). The transphosphorylations are reversible between Cr and CrP, an...