Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1

• Published in: Nature communications Vol. 7; no. 1; p. 13097
• Main Authors: Samata, Bumpei, Doi, Daisuke, Nishimura, Kaneyasu, Kikuchi, Tetsuhiro, Watanabe, Akira, Sakamoto, Yoshimasa, Kakuta, Jungo, Ono, Yuichi, Takahashi, Jun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.10.2016; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/100; 631/378/1689; 631/532/2064; 631/532/2182; 692/308/2171; Animals; Antibodies; Brain research; Cell Differentiation - genetics; Cell Separation - methods; Cells, Cultured; Dopaminergic Neurons - cytology; Dopaminergic Neurons - metabolism; Female; Gene expression; Human Embryonic Stem Cells - metabolism; Humanities and Social Sciences; Humans; Induced Pluripotent Stem Cells - metabolism; Macaca fascicularis; Male; Mesencephalon - cytology; Mesencephalon - metabolism; Mice, Inbred C57BL; multidisciplinary; Parkinson Disease - genetics; Parkinson Disease - metabolism; Parkinson Disease - therapy; Parkinson's disease; Proteins - genetics; Proteins - metabolism; Rats, Sprague-Dawley; Research centers; Science; Science (multidisciplinary); Serine Endopeptidases - genetics; Serine Endopeptidases - metabolism; Stem Cell Transplantation - methods; Stem cells; Transcription factors; Transplantation, Heterologous; Japan

Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (mDA) neurons for cell replacement therapy for Parkinson’s disease (PD). However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. To eliminate these unwanted cells, ce...