High Diversity and Abundance of Legionella spp. in a Pristine River and Impact of Seasonal and Anthropogenic Effects

• Published in: Applied and Environmental Microbiology Vol. 76; no. 24; pp. 8201 - 8210
• Main Authors: Parthuisot, N, West, N.J, Lebaron, P, Baudart, J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.2010; American Society for Microbiology (ASM)
• Subjects: anthropogenic activities; Anthropogenic factors; Bacteria; Bacterial Load; Biological and medical sciences; Cluster Analysis; Culture media; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Ecological studies; Environmental Microbiology; Environmental Sciences; France; Freshwater environments; Fundamental and applied biological sciences. Psychology; Genes; Genetic Variation; Human Activities; Legionella - classification; Legionella - genetics; Legionella - isolation & purification; Legionella pneumophila; Microbiology; Molecular Sequence Data; monitoring; nucleotide sequences; Phylogeny; polymerase chain reaction; Polymorphism; Ribonucleic acid; ribosomal RNA; Rivers; Rivers - microbiology; RNA; RNA, Ribosomal, 16S - genetics; Seasons; Sequence Analysis, DNA; single-stranded conformational polymorphism; Wastewater discharges; Water treatment; Watersheds; France; Bacteria; Abundance; Legionella; Rivers; Legionellaceae; Diversity

The diversity and dynamics of Legionella species along a French river watershed subject to different thermal and wastewater discharges during an annual cycle were assessed by 16S rRNA gene sequencing and by a fingerprint technique, single-strand conformation polymorphism. A high diversity of Legione...