张应力刺激下Wnt／β-catenin信号通路对成牙骨质细胞Runx2表达调控的体外研究
目的研究张应力刺激下Wnt/β-catenin信号通路对成牙骨质细胞Runx2表达的调控。方法以成牙骨质细胞样细胞OCCM30为研究对象,应用FX4000T应力加载装置对其加载张应力,时间为0、3、6、12 h,然后分别使用不同质量浓度的Dikkopf-1（DKK1）处理48 h,采用Western blot法检测β-catenin蛋白的表达水平,实时荧光定量聚合酶链反应法（RT-PCR）检测Runx2 mRNA的表达水平。选取最佳作用时间点（12 h）和最佳阻断剂质量浓度（150 ng·m L^-1）,将样本分为A、B、C、D共4组,A组不加力不加阻断剂,B组加力加阻断剂,C组加力不加阻断剂...
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| Published in | 华西口腔医学杂志 Vol. 33; no. 1; pp. 35 - 39 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
重庆医科大学附属口腔医院正畸科，口腔疾病与生物医学重庆市重点实验室，重庆 401147
2015
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-1182 |
| DOI | 10.7518/hxkq.2015.01.008 |
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| Abstract | 目的研究张应力刺激下Wnt/β-catenin信号通路对成牙骨质细胞Runx2表达的调控。方法以成牙骨质细胞样细胞OCCM30为研究对象,应用FX4000T应力加载装置对其加载张应力,时间为0、3、6、12 h,然后分别使用不同质量浓度的Dikkopf-1（DKK1）处理48 h,采用Western blot法检测β-catenin蛋白的表达水平,实时荧光定量聚合酶链反应法（RT-PCR）检测Runx2 mRNA的表达水平。选取最佳作用时间点（12 h）和最佳阻断剂质量浓度（150 ng·m L^-1）,将样本分为A、B、C、D共4组,A组不加力不加阻断剂,B组加力加阻断剂,C组加力不加阻断剂,D组不加力加阻断剂,测量β-catenin蛋白和Runx2 mRNA的表达水平。结果 1）张应力加载3、6、12 h后,Runx2 mRNA表达水平和β-catenin蛋白水平与对照组相比均明显升高,差异有统计学意义（P〈0.05）。2）不同质量浓度DKK1处理后,细胞内Runx2 mRNA的表达量呈下降趋势。3）未加载张应力的情况下,DKK1明显抑制了β-catenin蛋白的表达,Wnt/β-catenin通路被抑制;加载张应力情况下,DKK1处理组β-catenin蛋白表达低于未加DKK1处理组;不加阻断剂的情况下,张应力刺激增强了β-catenin蛋白的表达;阻断剂作用后,应力刺激组β-catenin蛋白表达相对较高。结论机械应力刺激下Wnt/β-catenin信号通路可正向调控成牙骨质细胞Runx2基因的表达。 |
|---|---|
| AbstractList | 目的研究张应力刺激下Wnt/β-catenin信号通路对成牙骨质细胞Runx2表达的调控。方法以成牙骨质细胞样细胞OCCM30为研究对象,应用FX4000T应力加载装置对其加载张应力,时间为0、3、6、12 h,然后分别使用不同质量浓度的Dikkopf-1（DKK1）处理48 h,采用Western blot法检测β-catenin蛋白的表达水平,实时荧光定量聚合酶链反应法（RT-PCR）检测Runx2 mRNA的表达水平。选取最佳作用时间点（12 h）和最佳阻断剂质量浓度（150 ng·m L^-1）,将样本分为A、B、C、D共4组,A组不加力不加阻断剂,B组加力加阻断剂,C组加力不加阻断剂,D组不加力加阻断剂,测量β-catenin蛋白和Runx2 mRNA的表达水平。结果 1）张应力加载3、6、12 h后,Runx2 mRNA表达水平和β-catenin蛋白水平与对照组相比均明显升高,差异有统计学意义（P〈0.05）。2）不同质量浓度DKK1处理后,细胞内Runx2 mRNA的表达量呈下降趋势。3）未加载张应力的情况下,DKK1明显抑制了β-catenin蛋白的表达,Wnt/β-catenin通路被抑制;加载张应力情况下,DKK1处理组β-catenin蛋白表达低于未加DKK1处理组;不加阻断剂的情况下,张应力刺激增强了β-catenin蛋白的表达;阻断剂作用后,应力刺激组β-catenin蛋白表达相对较高。结论机械应力刺激下Wnt/β-catenin信号通路可正向调控成牙骨质细胞Runx2基因的表达。 R783.3; 目的：研究张应力刺激下Wnt/β-catenin信号通路对成牙骨质细胞Runx2表达的调控。方法以成牙骨质细胞样细胞OCCM30为研究对象，应用FX4000T应力加载装置对其加载张应力，时间为0、3、6、12 h，然后分别使用不同质量浓度的Dikkopf-1（DKK1）处理48 h，采用Western blot法检测β-catenin蛋白的表达水平，实时荧光定量聚合酶链反应法（RT-PCR）检测Runx2 mRNA的表达水平。选取最佳作用时间点（12 h）和最佳阻断剂质量浓度（150 ng·mL-1），将样本分为A、B、C、D共4组，A组不加力不加阻断剂，B组加力加阻断剂，C组加力不加阻断剂，D组不加力加阻断剂，测量β-catenin蛋白和Runx2 mRNA的表达水平。结果1）张应力加载3、6、12 h后， Runx2 mRNA表达水平和β-catenin蛋白水平与对照组相比均明显升高，差异有统计学意义（P<0.05）。2）不同质量浓度DKK1处理后，细胞内Runx2 mRNA的表达量呈下降趋势。3）未加载张应力的情况下，DKK1明显抑制了β-catenin蛋白的表达，Wnt/β-catenin通路被抑制；加载张应力情况下，DKK1处理组β-catenin蛋白表达低于未加DKK1处理组；不加阻断剂的情况下，张应力刺激增强了β-catenin蛋白的表达；阻断剂作用后，应力刺激组β-catenin蛋白表达相对较高。结论机械应力刺激下Wnt/β-catenin信号通路可正向调控成牙骨质细胞Runx2基因的表达。 |
| Author | 李书琴 杨珊 任嫒姝 戴红卫 |
| AuthorAffiliation | 重庆医科大学附属口腔医院正畸科,口腔疾病与生物医学重庆市重点实验室,重庆401147 |
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| ClassificationCodes | R783.3 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.7518/hxkq.2015.01.008 |
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| DocumentTitleAlternate | Investigation of Wnt/β-catenin signaling pathway on regulation of Runx2 in cementoblasts under mechanical stress invitro |
| EndPage | 39 |
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| Keywords | 牙根吸收 牙根修复 成牙骨质细胞 Wnt/β-catenin Runx2 |
| Language | Chinese |
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| Notes | Objective Periodontal tissue remodeling includes remodeling of alveolar bone, periodontal ligament, and cementum. Cementoblast plays a main role in repairing root resorption. Canonical Wnt/β-catenin signaling can promote the odontogenic differentiation in osteoblast. However, the mechanism on how the orthodontic force influences the function of cementoblast and the relationship between the canonical Wnt/β-catenin signaling and Rtmx2 of cementoblast are＇not yet known. The aim of this study is focus on this relationship. Methods OCCM30 cementoblasts were subjected to mechanical strain by four-point bending system with tension stress for 0, 3, 6, and 12 h. They were pretreated with different concentrations of Dikkopf-1 （DKK1） for 48 h. Western blot analysis was performed to detect the β-catenin levels in the nucleus. Rtmx2 mRNA was observed by real-time quantitative polymerase chain reaction （RT-PCR）. OCCM30 cementoblasts were then pretreated with 150 ng·mL^-1 DKK1 for 48 h and subjected to mechanical strain by |
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| PublicationTitle | 华西口腔医学杂志 |
| PublicationTitleAlternate | West China Journal of Stomatology |
| PublicationTitle_FL | West China Journal of Stomatology |
| PublicationYear | 2015 |
| Publisher | 重庆医科大学附属口腔医院正畸科，口腔疾病与生物医学重庆市重点实验室，重庆 401147 |
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| Snippet | 目的研究张应力刺激下Wnt/β-catenin信号通路对成牙骨质细胞Runx2表达的调控。方法以成牙骨质细胞样细胞OCCM30为研究对象,应用FX4000T应力加载装置对其加载张应力,时间为0、3、6、12 h,然后分别使用不同质量浓度的Dikkopf-1（DKK1）处理48 h,采用Western... R783.3; 目的：研究张应力刺激下Wnt/β-catenin信号通路对成牙骨质细胞Runx2表达的调控。方法以成牙骨质细胞样细胞OCCM30为研究对象，应用FX4000T应力加载装置对其加载张应力，时间为0、3、6、12 h，然后分别使用不同质量浓度的Dikkopf-1（DKK1）处理48 h，采用Western... |
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| StartPage | 35 |
| SubjectTerms | Runx2 Wnt／β-catenin 成牙骨质细胞 牙根修复 牙根吸收 |
| Title | 张应力刺激下Wnt／β-catenin信号通路对成牙骨质细胞Runx2表达调控的体外研究 |
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