A comparative evaluation of the analytical performances of premier resolution-high-performance liquid chromatography (PR-HPLC) with capillary zone electrophoresis (CZE) assays for the detection of hemoglobin variants and the quantitation of HbA 0 , A 2 , E, and F

• Published in: Clinical chemistry and laboratory medicine Vol. 62; no. 7; pp. 1383 - 1392
• Main Authors: Laksap, Sirikwan, Suanboon, Suphisara, Punyamung, Manoo, Ruengdit, Chedtapak, Pornprasert, Sakorn
• Format: Journal Article
• Language: English
• Published: Germany 25.06.2024
• Subjects: Adult; Chromatography, High Pressure Liquid - methods; Electrophoresis, Capillary - methods; Fetal Hemoglobin - analysis; Hemoglobin A2 - analysis; Hemoglobin E - analysis; Hemoglobinopathies - blood; Hemoglobinopathies - diagnosis; Hemoglobins, Abnormal - analysis; Humans; capillary zone electrophoresis; high-performance liquid chromatography; thalassemia; hemoglobin analysis; hemoglobinopathy; premier resolution

Hemoglobinopathies, including thalassemia and hemoglobin (Hb) variants, are common hematological disorders in tropical countries. Accurate and precise separation of hemoglobin types and reliable quantitation are necessary for differential diagnosis of these disorders. We have evaluated the analytica...