Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS

• Published in: PloS one Vol. 9; no. 10; p. e110127
• Main Authors: Branquinho, Raquel, Sousa, Clara, Lopes, João, Pintado, Manuela E., Peixe, Luísa V., Osório, Hugo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.10.2014; Public Library of Science (PLoS)
• Subjects: Bacillus; Bacillus - classification; Bacillus - genetics; Bacillus cereus; Bacillus pumilus; Bacillus safensis; Bacillus subtilis; Bacterial Proteins; Bacterial Typing Techniques; Biology and Life Sciences; Biomarkers; Biopesticides; Biotechnology; Chemometrics; Computer programs; Deoxyribonucleic acid; Desorption; Differentiation; Discriminant analysis; DNA; E coli; Escherichia coli; Food; Genes; Identification; Ionization; Ions; Lasers; Mass spectra; Mass spectrometry; Mass spectroscopy; Microorganisms; Physical Sciences; Proteins; Research and Analysis Methods; RNA polymerase; Scientific imaging; Species; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; United States > US; Portugal

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. Bacillus pumillus and Bacillus safensis, a...