Genome-wide antisense transcription drives mRNA processing in bacteria

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 50; pp. 20172 - 20177
• Main Authors: Lasa, Iñigo, Toledo-Arana, Alejandro, Dobin, Alexander, Villanueva, Maite, de los Mozos, Igor Ruiz, Vergara-Irigaray, Marta, Segura, Víctor, Fagegaltier, Delphine, Penadés, José R, Valle, Jaione, Solano, Cristina, Gingeras, Thomas R
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 13.12.2011; National Acad Sciences
• Subjects: 3' untranslated regions; Antisense RNA; Bacteria; Biological Sciences; digestion; Gene Expression Regulation, Bacterial; Genes; Genome, Bacterial - genetics; Genomes; Gram-positive bacteria; high-throughput nucleotide sequencing; Humans; Libraries; Messenger RNA; Molecules; Open reading frames; Open Reading Frames - genetics; Operator regions; Ribonuclease III - metabolism; ribonucleases; Ribonucleic acid; RNA; RNA Processing, Post-Transcriptional - genetics; RNA, Antisense - genetics; RNA, Antisense - metabolism; RNA, Bacterial - genetics; RNA, Double-Stranded - genetics; RNA, Double-Stranded - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Analysis, RNA; Species Specificity; Staphylococcus aureus; Staphylococcus aureus - genetics; Staphylococcus infections; Transcription, Genetic; transcriptome; Transcriptomes

RNA deep sequencing technologies are revealing unexpected levels of complexity in bacterial transcriptomes with the discovery of abundant noncoding RNAs, antisense RNAs, long 5' and 3' untranslated regions, and alternative operon structures. Here, by applying deep RNA sequencing to both th...