Stabilization of a Bimolecular Triplex by 3′-S-Phosphorothiolate Modifications: An NMR and UV Thermal Melting Investigation

• Published in: Chemistry : a European journal Vol. 21; no. 19; pp. 7278 - 7284
• Main Authors: Evans, Kathryn, Bhamra, Inder, Wheelhouse, Richard T., Arnold, John R. P., Cosstick, Richard, Fisher, Julie
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 04.05.2015; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: 3′-S-phosphorothiolate; Binding; Biological; Chemistry; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA structures; Grooves; Melting; Models, Molecular; Molecular biology; NMR spectroscopy; Nuclear Magnetic Resonance, Biomolecular; Nucleic Acid Conformation; Nucleic Acid Denaturation; Nucleic acids; Phosphates - chemistry; Ribonucleic acids; Stabilization; Strands; Thermodynamics; UV thermal melting; UV thermal melting; nucleic acids; NMR spectroscopy; 3′-S-phosphorothiolate; DNA structures

Triplexes formed from oligonucleic acids are key to a number of biological processes. They have attracted attention as molecular biology tools and as a result of their relevance in novel therapeutic strategies. The recognition properties of single‐stranded nucleic acids are also relevant in third‐st...