Profiling allergic asthma volatile metabolic patterns using a headspace-solid phase microextraction/gas chromatography based methodology

• Published in: Journal of Chromatography A Vol. 1218; no. 24; pp. 3771 - 3780
• Main Authors: Caldeira, M., Barros, A.S., Bilelo, M.J., Parada, A., Câmara, J.S., Rocha, S.M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 17.06.2011; Elsevier
• Subjects: acids; Adolescent; aldehydes; alkanes; Allergic asthma; Analysis of Variance; aromatic hydrocarbons; asthma; Asthma - metabolism; bags; Biological and medical sciences; Breath Tests - methods; Case-Control Studies; Child; Child, Preschool; children; coatings; Exhaled breath; experimental design; Female; gas chromatography; Gas Chromatography-Mass Spectrometry - methods; Gas chromatography–mass spectrometry; General aspects; headspace analysis; Headspace solid-phase microextraction; Humans; Immunopathology; Investigative techniques, diagnostic techniques (general aspects); ketones; Male; mass spectrometry; Medical sciences; metabolites; Organic Chemicals - analysis; Organic Chemicals - isolation & purification; oxidative stress; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; public health; rapid methods; Respiratory system; rhinitis; solid phase microextraction; Solid Phase Microextraction - methods; temperature; volatile compounds; Volatile metabolites; Allergic asthma; Headspace solid-phase microextraction; Volatile metabolites; Gas chromatography–mass spectrometry; Exhaled breath; Allergy; Metabolomics; Nose disease; Chemical analysis; Rhinitis; Metabolite; Optimization; Quadrupole spectrometer; Chemical enrichment; Sample preparation; Clinical biology; ENT disease; Bronchus disease; Obstructive pulmonary disease; Child; Human; Trace analysis; Immunopathology; Lung disease; Expired air; Discriminant analysis; Statistical analysis; Healthy subject; Respiratory disease; Coupled method; Patient; Volatile organic compound; Gas chromatography-mass spectrometry; Asthma; Solid phase microextraction; Gas chromatography; PLS regression; Experimental design; Headspace; Mass spectrometry; Chemometrics

Allergic asthma represents an important public health issue with significant growth over the years, especially in the paediatric population. Exhaled breath is a non-invasive, easily performed and rapid method for obtaining samples from the lower respiratory tract. In the present manuscript, the meta...