High-performance liquid chromatographic analysis of bilirubin and biliverdin from jaundiced broilers

• Published in: Journal of Veterinary Medical Science Vol. 59; no. 8; pp. 677 - 680
• Main Authors: Mizobe, M. (Miyazaki Univ. (Japan). Faculty of Agriculture), Kondo, F, Kumamoto, K, Kanda, Y, Seguchi, H
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.08.1997; Japan Science and Technology Agency
• Subjects: Animals; BILIRRUBINA; BILIRUBIN; Bilirubin - analysis; BILIRUBINE; Biliverdine - analysis; broiler; BROILER CHICKENS; Chickens; Cholestasis - blood; Cholestasis - epidemiology; Cholestasis - veterinary; Chromatography, High Pressure Liquid - methods; DIAGNOSIS; DIAGNOSTIC; DIAGNOSTICO; Disease Outbreaks - veterinary; Hepatitis, Animal - diagnosis; Hepatitis, Animal - epidemiology; HPLC; Humans; ICTERE; ICTERICIA; Japan - epidemiology; JAUNDICE; Jaundice - diagnosis; Jaundice - epidemiology; Jaundice - veterinary; Liver - pathology; Pericardium; POLLO DE ENGORDE; POULET DE CHAIR; Poultry Diseases; Japan

A sensitive and rapid high-performance liquid chromatographic (HPLC) method was developed and used for the simultaneous determination of bilirubin and biliverdin in pericardial fluid samples collected from broilers at a poultry inspection site. A photodiode array detector distinguishing the bilirubi...