High-performance liquid chromatographic analysis of bilirubin and biliverdin from jaundiced broilers

A sensitive and rapid high-performance liquid chromatographic (HPLC) method was developed and used for the simultaneous determination of bilirubin and biliverdin in pericardial fluid samples collected from broilers at a poultry inspection site. A photodiode array detector distinguishing the bilirubi...

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Published inJournal of Veterinary Medical Science Vol. 59; no. 8; pp. 677 - 680
Main Authors Mizobe, M. (Miyazaki Univ. (Japan). Faculty of Agriculture), Kondo, F, Kumamoto, K, Kanda, Y, Seguchi, H
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.08.1997
Japan Science and Technology Agency
Subjects
Animals
BILIRRUBINA
BILIRUBIN
Bilirubin - analysis
BILIRUBINE
Biliverdine - analysis
broiler
BROILER CHICKENS
Chickens
Cholestasis - blood
Cholestasis - epidemiology
Cholestasis - veterinary
Chromatography, High Pressure Liquid - methods
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
Disease Outbreaks - veterinary
Hepatitis, Animal - diagnosis
Hepatitis, Animal - epidemiology
HPLC
Humans
ICTERE
ICTERICIA
Japan - epidemiology
JAUNDICE
Jaundice - diagnosis
Jaundice - epidemiology
Jaundice - veterinary
Liver - pathology
Pericardium
POLLO DE ENGORDE
POULET DE CHAIR
Poultry Diseases
Japan
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Abstract A sensitive and rapid high-performance liquid chromatographic (HPLC) method was developed and used for the simultaneous determination of bilirubin and biliverdin in pericardial fluid samples collected from broilers at a poultry inspection site. A photodiode array detector distinguishing the bilirubin (UV 450 nm) and biliverdin (365 nm) was used as an analytical detector for HPLC system. An internal-surface reversed-phase silica support column was used, and the mobile phase consisted of acetonitrile: 0.5 M Tris HCl buffer (20:80, pH 7.2). Bilirubin was detected from all of the jaundiced pericardial fluid samples, and a small amount of biliverdin was detected with bilirubin in some samples. These jaundiced broilers had hepatic or bile duct lesions similar to those found in edible animals. From these results, a working definition of jaundiced broilers for poultry inspection sites was suggested: bilirubin is detectable from pericardial fluid and the carcass is in a state of yellow color change.
AbstractList A sensitive and rapid high-performance liquid chromatographic (HPLC) method was developed and used for the simultaneous determination of bilirubin and biliverdin in pericardial fluid samples collected from broilers at a poultry inspection site. A photodiode array detector distinguishing the bilirubin (UV 450 nm) and biliverdin (365 nm) was used as an analytical detector for HPLC system. An internal-surface reversed-phase silica support column was used, and the mobile phase consisted of acetonitrile: 0.5 M Tris HCl buffer (20:80, pH 7.2). Bilirubin was detected from all of the jaundiced pericardial fluid samples, and a small amount of biliverdin was detected with bilirubin in some samples. These jaundiced broilers had hepatic or bile duct lesions similar to those found in edible animals. From these results, a working definition of jaundiced broilers for poultry inspection sites was suggested: bilirubin is detectable from pericardial fluid and the carcass is in a state of yellow color change.
Author Kondo, F
Kumamoto, K
Seguchi, H
Mizobe, M. (Miyazaki Univ. (Japan). Faculty of Agriculture)
Kanda, Y
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Cites_doi 10.1016/S0021-9673(01)83493-2
10.1016/S0034-5288(18)34612-5
10.1021/ac00285a058
10.1016/S0378-4347(00)83660-9
10.1292/jvms.58.495
10.1016/S0378-4347(00)86073-9
10.1042/bj2250787
10.1016/0003-2697(89)90478-8
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SubjectTerms Animals
BILIRRUBINA
BILIRUBIN
Bilirubin - analysis
BILIRUBINE
Biliverdine - analysis
broiler
BROILER CHICKENS
Chickens
Cholestasis - blood
Cholestasis - epidemiology
Cholestasis - veterinary
Chromatography, High Pressure Liquid - methods
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
Disease Outbreaks - veterinary
Hepatitis, Animal - diagnosis
Hepatitis, Animal - epidemiology
HPLC
Humans
ICTERE
ICTERICIA
Japan - epidemiology
JAUNDICE
Jaundice - diagnosis
Jaundice - epidemiology
Jaundice - veterinary
Liver - pathology
Pericardium
POLLO DE ENGORDE
POULET DE CHAIR
Poultry Diseases
Title High-performance liquid chromatographic analysis of bilirubin and biliverdin from jaundiced broilers
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