High-performance liquid chromatographic analysis of bilirubin and biliverdin from jaundiced broilers

• Published in: Journal of Veterinary Medical Science Vol. 59; no. 8; pp. 677 - 680
• Main Authors: Mizobe, M. (Miyazaki Univ. (Japan). Faculty of Agriculture), Kondo, F, Kumamoto, K, Kanda, Y, Seguchi, H
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.08.1997; Japan Science and Technology Agency
• Subjects: Animals; BILIRRUBINA; BILIRUBIN; Bilirubin - analysis; BILIRUBINE; Biliverdine - analysis; broiler; BROILER CHICKENS; Chickens; Cholestasis - blood; Cholestasis - epidemiology; Cholestasis - veterinary; Chromatography, High Pressure Liquid - methods; DIAGNOSIS; DIAGNOSTIC; DIAGNOSTICO; Disease Outbreaks - veterinary; Hepatitis, Animal - diagnosis; Hepatitis, Animal - epidemiology; HPLC; Humans; ICTERE; ICTERICIA; Japan - epidemiology; JAUNDICE; Jaundice - diagnosis; Jaundice - epidemiology; Jaundice - veterinary; Liver - pathology; Pericardium; POLLO DE ENGORDE; POULET DE CHAIR; Poultry Diseases; Japan
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.59.677

A sensitive and rapid high-performance liquid chromatographic (HPLC) method was developed and used for the simultaneous determination of bilirubin and biliverdin in pericardial fluid samples collected from broilers at a poultry inspection site. A photodiode array detector distinguishing the bilirubin (UV 450 nm) and biliverdin (365 nm) was used as an analytical detector for HPLC system. An internal-surface reversed-phase silica support column was used, and the mobile phase consisted of acetonitrile: 0.5 M Tris HCl buffer (20:80, pH 7.2). Bilirubin was detected from all of the jaundiced pericardial fluid samples, and a small amount of biliverdin was detected with bilirubin in some samples. These jaundiced broilers had hepatic or bile duct lesions similar to those found in edible animals. From these results, a working definition of jaundiced broilers for poultry inspection sites was suggested: bilirubin is detectable from pericardial fluid and the carcass is in a state of yellow color change.